Professor Andrew Bowie
Professor of Innate Immunology, Biochemistry
Head of School, Sch Office - Biochemistry & Immunology
Biography
Andrew Bowie obtained his PhD in Biochemistry from Trinity College Dublin in 1997. After postdoctoral training with Prof Luke O'Neill, he was appointed as lecturer in the Department of Pharmacology in UCD, before returning to TCD in 2001 to establish the first and only Immunology undergraduate degree course in Ireland, which he coordinated from 2002 - 2006. He served as Director of Research in the School of Biochemistry and Immunology from 2005 - 2009, and as Head of Immunology from 2011 - 2017. In 2008 he was elected a Fellow of TCD, and in 2014 a member of the Royal Irish Academy. He is actively involved in both undergraduate and postgraduate Immunology teaching, and has graduated 14 PhD students to date. His research discoveries and scholarly activities have significantly contributed to TCD's global reputation as a place of excellence for Immunology teaching and research and in 2017 he was awarded the Irish Society for Immunology Public Lecture Medal for his outstanding contribution to Irish Immunology. He is a global leader in the research fields of innate immunity and viral evasion, and his research occupies a unique space at the interface between virology, immunology and disease pathogenesis. He has raised research funding of >9m (direct costs to PI) since 2001, from national and international funding agencies including Science Foundation Ireland, the EU and the US National Institutes of Health, and also from private industry. Work in his laboratory has shed light on how pattern recognition receptors (PRRs) recognise pathogens, leading to the induction of interferons and cytokines, which control infection locally as well as coordinating the adaptive immune response. He also investigates how PRRs and other innate immune sensors drive inflammation, and discovered one of the key cellular sensors of DNA in 2010, IFI16, which has subsequently been shown to be critical in understanding viral pathogenesis of diverse viruses including HIV and herpes viruses. He has recently identified novel roles for a number of innate immune proteins in regulating inflammation via cytokine modulation, and is currently exploring their mechanisms of action. His seminal discoveries have redefined our understanding of how pathogens engage with human cells, and he has regularly published research papers, opinion pieces and reviews in leading international journals including Nature Immunology, Immunity, The Journal of Experimental Medicine, Nature Reviews Immunology, PNAS, PLoS Pathogens and The EMBO Journal.
Publications and Further Research Outputs
Peer-Reviewed Publications
Huang, Y. and Bergant, V. and Grass, V. and Emslander, Q. and Hamad, M.S. and Hubel, P. and Mergner, J. and Piras, A. and Krey, K. and Henrici, A. and Ã-llinger, R. and Tesfamariam, Y.M. and Dalla Rosa, I. and Bunse, T. and Sutter, G. and Ebert, G. and Schmidt, F.I. and Way, M. and Rad, R. and Bowie, A.G. and Protzer, U. and Pichlmair, A., Multi-omics characterization of the monkeypox virus infection, Nature Communications, 15, (1), 2024
Gewaid H, Bowie AG., Regulation of type I and type III interferon induction in response to pathogen sensing., Current opinion in immunology, 87, 2024, p102424
Martiáñez-Vendrell, X. and Bloeme-ter Horst, J. and Hutchinson, R. and Guy, C. and Bowie, A.G. and Kikkert, M., Human Coronavirus 229E Infection Inactivates Pyroptosis Executioner Gasdermin D but Ultimately Leads to Lytic Cell Death Partly Mediated by Gasdermin E, Viruses, 16, (6), 2024
Al Hamrashdi, M. and Perez, C.S. and Haas, D.A. and Vishwakarma, J. and Pichlmair, A. and Bowie, A.G. and Brady, G., Molluscum contagiosum virus protein MC089 inhibits interferon regulatory factor 3 activation, Journal of General Virology, 105, (8), 2024
Joanna L. Turley, Ross W. Ward, Jorge Huete-Carrasco, Natalia Muñoz-Wolf, Kate Roche, Lei Jin, Andrew Bowie, Mats Andersson, Ed Lavelle, Intratumoral delivery of the chitin-derived C100 adjuvant promotes robust STING, IFNAR, and CD8+ T cell-dependent anti-tumor immunity, Cell Reports Medicine, 5, (5), 2024, p101560
Shanahan KA, Davis GM, Doran CG, Sugisawa R, Davey GP, Bowie AG., SARM1 regulates NAD+-linked metabolism and select immune genes in macrophages., The Journal of Biological Chemistry, 300, (2), 2024, p105620
Ryoichi Sugisawa, Katharine A. Shanahan, Gavin M. Davis, Gavin P. Davey, Andrew Bowie, SARM1 regulates pro-inflammatory cytokine expression in human monocytes by NADase-dependent and -independent mechanisms, iScience, 27, (6), 2024, p109940
Baran, M. and Feriotti, C. and McGinley, A. and Carlile, S.R. and Jiang, Z. and Calderon-Gonzalez, R. and Dumigan, A. and Sá-Pessoa, J. and Sutton, C.E. and Kearney, J. and McLoughlin, R.M. and Mills, K.H.G. and Fitzgerald, K.A. and Bengeochea, J.A. and Bowie, A.G., PYHIN protein IFI207 regulates cytokine transcription and IRF7 and contributes to the establishment of K. pneumoniae infection, Cell Reports, 42, (4), 2023
Phelan, T. and Lawler, C. and Pichlmair, A. and Little, M.A. and Bowie, A.G. and Brady, G., Molluscum Contagiosum Virus Protein MC008 Targets NF-kB Activation by Inhibiting Ubiquitination of NEMO, Journal of Virology, 97, (3), 2023
Coralie Guy, Marcin Baran, Pau Ribó-Molina, Bernadette G van den Hoogen, Andrew G Bowie, Viral sensing by epithelial cells involves PKR- and caspase-3-dependent generation of gasdermin E pores, iScience, 2023
Muñoz-Wolf, N. and Ward, R.W. and Hearnden, C.H. and Sharp, F.A. and Geoghegan, J. and O'Grady, K. and McEntee, C.P. and Shanahan, K.A. and Guy, C. and Bowie, A.G. and Campbell, M. and Roces, C.B. and Anderluzzi, G. and Webb, C. and Perrie, Y. and Creagh, E. and Lavelle, E.C., Non-canonical inflammasome activation mediates the adjuvanticity of nanoparticles, Cell Reports Medicine, 4, (1), 2023
Claudia Feriotti, Joana Sa-Pessoa, Ricardo Calderon-Gonzalez, Lili Gu, Brenda Morris, Ryoichi Sugisawa, Jose L Insua, Michael Carty, Amy Dumigan, Rebecca J Ingram, Adrien Kissenpfening, Andrew G Bowie, Jose A Bengoechea, Klebsiella pneumoniae hijacks the Toll-IL-1R protein SARM1 in a type I IFN-dependent manner to antagonize host immunity., Cell Reports, 40, (6), 2022, p111167-
Singh S, O'Reilly S, Gewaid H, Bowie AG, Gautier V, Worrall DM., Reactive Centre Loop Mutagenesis of SerpinB3 to Target TMPRSS2 and Furin: Inhibition of SARS-CoV-2 Cell Entry and Replication., International journal of molecular sciences, 23, (20), 2022, p12522
Gibbons, L. and Ozaki, E. and Greene, C. and Trappe, A. and Carty, M. and Coppinger, J.A. and Bowie, A.G. and Campbell, M. and Doyle, S.L., SARM1 Promotes Photoreceptor Degeneration in an Oxidative Stress Model of Retinal Degeneration, Frontiers in Neuroscience, 16, (852114), 2022
Guy, C. and Bowie, A.G., Recent insights into innate immune nucleic acid sensing during viral infection, Current Opinion in Immunology, 78, (102250), 2022
Laura K Finnegan, Naomi Chadderton, Paul F Kenna, Arpad Palfi, Michael Carty, Andrew G Bowie, Sophia Millington-Ward, Gwyneth Jane Farrar, SARM1 Ablation Is Protective and Preserves Spatial Vision in an In Vivo Mouse Model of Retinal Ganglion Cell Degeneration, International Journal of Molecular Sciences, 2022
McElroy AN, Invernizzi R, Laskowska JW, O'Neill A, Doroudian M, Moghoofei M, Mostafaei S, Li F, Przybylski AA, O'Dwyer DN, Bowie AG, Fallon PG, Maher TM, Hogaboam CM, Molyneaux PL, Hirani N, Armstrong ME, Donnelly SC., Candidate Role for Toll-like Receptor 3 L412F Polymorphism and Infection in Acute Exacerbation of Idiopathic Pulmonary Fibrosis., American journal of respiratory and critical care medicine, 2022
Carty M, Guy C, Bowie AG., Detection of Viral Infections by Innate Immunity., Biochemical pharmacology, 183, 2021, p114316
Feng Gu, Aileen Krüger, Hannes G Roggenkamp, Rick Alpers, Dmitri Lodygin, Vincent Jaquet, Franziska Möckl, Lola C Hernandez C, Kai Winterberg, Andreas Bauche, Anette Rosche, Helmut Grasberger, John Y Kao, Daniel Schetelig, René Werner, Katrin Schröder, Michael Carty, Andrew G Bowie, Samuel Huber, Chris Meier, Hans-Willi Mittrücker, Joerg Heeren, Karl-Heinz Krause, Alexander Flügel, Björn-Philipp Diercks, Andreas H Guse, Dual NADPH oxidases DUOX1 and DUOX2 synthesize NAADP and are necessary for Ca2+ signaling during T cell activation, Science Signaling, 14, (709), 2021
Ofir-Birin Y, Ben Ami Pilo H, Cruz Camacho A, Rudik A, Rivkin A, Revach OY, Nir N, Block Tamin T, Abou Karam P, Kiper E, Peleg Y, Nevo R, Solomon A, Havkin-Solomon T, Rojas A, Rotkopf R, Porat Z, Avni D, Schwartz E, Zillinger T, Hartmann G, Di Pizio A, Quashie NB, Dikstein R, Gerlic M, Torrecilhas AC, Levy C, Nolte-'t Hoen ENM, Bowie AG, Regev-Rudzki N., Malaria parasites both repress host CXCL10 and use it as a cue for growth acceleration., Nature communications, 12, (1), 2021, p4851
Ciara G Doran, Ryoichi Sugisawa, Michael Carty, Fiona Roche, Claire Fergus, Karsten Hokamp, Vincent P Kelly, Andrew G Bowie, CRISPR/Cas9-mediated SARM1 knockout and epitope-tagged mice reveal that SARM1 does not regulate nuclear transcription, but is expressed in macrophages, Journal of Biological Chemistry, 297, (6), 2021
Ozaki, Ema, Gibbons, Luke, Neto, Nuno GB, Kenna, Paul, Carty, Michael, Humphries, Marian, Humphries, Pete, Campbell, Matthew, Monaghan, Michael, Bowie, Andrew and Doyle, Sarah L, SARM1 deficiency promotes rod and cone photoreceptor cell survival in a model of retinal degeneration, Life Science Alliance, 3, (5), 2020
van den Hoogen, B. and Santoni, A. and Sciumé, G. and Bowie, A. and O'Farrelly, C. and O'Neill, L. and Anthonsen, M. and Pardali, K. and Young, S. and Bergthaler, A. and Manel, N. and Zahn, R. and Kikkert, M. and Snijder, E. and van Kuppeveld, F. and Fouchier, R. and Hiscott, J., Immunometabolism pathways as the basis for innovative anti-viral strategies (INITIATE): A Marie Sklodowska-Curie innovative training network, Virus Research, 287, (198094), 2020
Massa, D. and Baran, M. and Bengoechea, J.A. and Bowie, A.G. and Voelker, D.R., PYHIN1 regulates pro-inflammatory cytokine induction rather than innate immune DNA sensing in airway epithelial cells, Journal of Biological Chemistry, 295, (14), 2020, p4438-4450
Bowie, A.G., Harnessing poxviral know-how for anti-cytokine therapies, Journal of Biological Chemistry, 294, (13), 2019, p5228-5229
Carty, M. and Kearney, J. and Shanahan, K.A. and Hams, E. and Sugisawa, R. and Connolly, D. and Doran, C.G. and Muñoz-Wolf, N. and GÃ"rtler, C. and Fitzgerald, K.A. and Lavelle, E.C. and Fallon, P.G. and Bowie, A.G., Cell Survival and Cytokine Release after Inflammasome Activation Is Regulated by the Toll-IL-1R Protein SARM, Immunity, 50, (6), 2019, p1412-1424.e6
Musilova, J. and Mulcahy, M.E. and Kuijk, M.M. and McLoughlin, R.M. and Bowie, A.G., Toll-like receptor 2-dependent endosomal signaling by Staphylococcus aureus in monocytes induces type i interferon and promotes intracellular survival, Journal of Biological Chemistry, 294, (45), 2019, p17031-17042
Carty M, Bowie AG., SARM: from immune regulator to cell executioner., Biochemical pharmacology, 2019, p52-62
Bowie, A.G., Self-RNA sentinels signal viral invasion news-and-views, Nature Immunology, 19, (1), 2018, p4-5
Cooke G, Kamal I, Strengert M, Hams E, Mawhinney L, Tynan A, O' Reilly C, O' Dwyer DN, Kunkel SL, Knaus UG, Shields DC, Moller DR, Bowie AG, Fallon PG, Hogaboam CM, Armstrong ME, Donnelly SC., Toll-like receptor 3 L412F polymorphism promotes a persistent clinical phenotype in pulmonary sarcoidosis., QJM, 2018, p217-224
Dempsey A, Keating SE, Carty M, Bowie AG., Poxviral protein E3-altered cytokine production reveals that DExD/H-box helicase 9 controls Toll-like receptor-stimulated immune responses., The Journal of biological chemistry, 293, (39), 2018, p14989-15001
Dunphy G, Flannery SM, Almine JF, Connolly DJ, Paulus C, Jønsson KL, Jakobsen MR, Nevels MM, Bowie AG, Unterholzner L., Non-canonical Activation of the DNA Sensing Adaptor STING by ATM and IFI16 Mediates NF-"B Signaling after Nuclear DNA Damage., Molecular cell, 71, (5), 2018, p745-760.e5
Sisquella, X. and Ofir-Birin, Y. and Pimentel, M.A. and Cheng, L. and Abou Karam, P. and Sampaio, N.G. and Penington, J.S. and Connolly, D. and Giladi, T. and Scicluna, B.J. and Sharples, R.A. and Waltmann, A. and Avni, D. and Schwartz, E. and Schofield, L. and Porat, Z. and Hansen, D.S. and Papenfuss, A.T. and Eriksson, E.M. and Gerlic, M. and Hill, A.F. and Bowie, A.G. and Regev-Rudzki, N., Malaria parasite DNA-harbouring vesicles activate cytosolic immune sensors, Nature Communications, 8, (1), 2017, p1985-
Almine J., Almine J., O'Hare C., O'Hare C., Dunphy G., Dunphy G., Haga I., Naik R., Atrih A., Connolly D., Taylor J., Kelsall I., Bowie A., Beard P., Beard P., Unterholzner L., Unterholzner L., IFI16 and cGAS cooperate in the activation of STING during DNA sensing in human keratinocytes, Nature Communications, 8, 2017, p14392
Mahony R, Gargan S, Roberts KL, Bourke N, Keating SE, Bowie AG, O'Farrelly C, Stevenson NJ, A novel anti-viral role for STAT3 in IFN-alpha signalling responses. , Cellular and Molecular Life Sciences, 74, (9), 2017, p1755 - 1764
Brady, G., Haas, D.A., Farrell, P.F., Pichlmair, A. and Bowie, A.G. , Molluscum Contagiosum Virus protein MC005 inhibits NFκB activation by targeting NEMO-regulated IKK activation, The Journal of Virology, 91, (15), 2017, p00545-17
Harte, C., Gorman, A.L., McCluskey, S., Carty, M., Bowie, A.G., Scott, C.J., Meade, K.G. and Lavelle, E.C., Alum activates the bovine NLRP3 inflammasome, Frontiers in Immunology, 8, 2017, p1494-
Shirey, K.A, Lai, W., Pletneva, L.M., Patel, M., Pang, C., Kurt-Jones, E., Lipsky, M., Roger, T., Calandra, T., Tracey, K., Al Abed, Y., Bowie, A.G., Fasano, A., Dinarello, C., Gusovsky, F., Blanco, J.C.G., and Vogel S.N., Novel Strategies for Targeting Innate Immune Responses to Influenza , Mucosal Immunology , 9, 2016, p1173 - 1182
Carroll, E.C., Jin, L., Mori, A., Muñoz-Wolf, N., Oleszycka, E., Moran, H.B.T., Mansouri, S., McEntee, C.P., Lambe, E., Agger, E.M., Andersen, P., Cunningham, C., Hertzog, P., Fitzgerald, K.A., Bowie, A.G., Lavelle, E.C., The Vaccine Adjuvant Chitosan Promotes Cellular Immunity via DNA Sensor cGAS-STING-Dependent Induction of Type I Interferons, Immunity, 44, (3), 2016, p597 - 608
Brady G, Boggan L, Bowie A, O'Neill L.A.J, Erratum: Schlafen-1 causes a cell cycle arrest by inhibiting induction of cyclin D1 (The Journal of Biological Chemistry (2005) 280 (30723-30734) DOI: 10.1074/jbc.M500435200), Journal of Biological Chemistry, 291, (48), 2016, p25278-
Wang, H., El Maadidi, S., Fischer, J., Grabski, E., Dickhöfer, S., Klimosch, S., Flannery, S.M., Filomena, A., Wolz, O-O., Schneiderhan-Marra, N., Löffler, M.W., Wiese, M., Pichulik, T., Müllhaupt, B., Semela, D., Dufour, J-F., the East-German and Swiss Hepatitis C Virus Study Groups, Bochud, P-Y., Bowie, A.G., Kalinke, U., Berg, T., Weber, A.N.R. , A frequent hypofunctional IRAK2 variant is associated with reduced spontaneous hepatitis C virus clearance, Hepatology, 62, 2015, p1375 - 1387
Cox, D. J., Field, R. H., Williams, D. G., Baran, M., Bowie, A. G., Cunningham, C. and Dunne, A. , DNA sensors are expressed in astrocytes and microglia in vitro and are upregulated during gliosis in neurodegenerative disease, GLIA, 63, (5), 2015, p812-825
Brady G, Haas DA, Farrell PJ, Pichlmair A, Bowie AG, Poxvirus Protein MC132 from Molluscum Contagiosum Virus Inhibits NF-B Activation by Targeting p65 for Degradation., Journal of virology, 89, (16), 2015, p8406-15
Dempsey A, Bowie AG, Innate immune recognition of DNA: A recent history., Virology, 479-480, 2015, p146-52
Viral Infections and the DNA Sensing Pathway: Lessons from Herpesviruses and Beyond in, editor(s)Ishii, K.J. and Tang, J.C.T. , Biological DNA Sensor: the Impact of Nucleic Acids on Diseases and Vaccinology, Elsevier, 2014, pp171 - 203, [Paludan, S.R. & Bowie, A.G. ]
Gürtler, C., Carty, M., Kearney, J., Schattgen, S.A., Ding, A., Fitzgerald, K.A. and Bowie, A.G. , SARM regulates CCL5 production in macrophages by promoting the recruitment of transcription factors and RNA polymerase II to the Ccl5 promoter, Journal of Immunology, 192, 2014, p4821 - 4832
Verstak B, Stack J, Ve T, Mangan M, Hjerrild K, Jeon J, Stahl R, Latz E, Gay N, Kobe B, Bowie AG, Mansell A, The TLR signaling adaptor TRAM interacts with TRAF6 to mediate activation of the inflammatory response by TLR4., Journal of leukocyte biology, 96, (3), 2014, p427-36
Stack J, Doyle SL, Connolly DJ, Reinert LS, O'Keeffe KM, McLoughlin RM, Paludan SR, Bowie AG, TRAM is required for TLR2 endosomal signaling to type I IFN induction., Journal of immunology (Baltimore, Md. : 1950), 193, (12), 2014, p6090-102
Andrew Bowie, Barry Harrington, Tatyana Lysakova, Brian Keogh, 'Vaccinia virus protein A46 peptide and use thereof', EPO, US Patent No. 8,722,052, 2014
Wang H, Flannery SM, Dickhöfer S, Huhn S, George J, Kubarenko AV, Lascorz J, Bevier M, Willemsen J, Pichulik T, Schafmayer C, Binder M, Manoury B, Paludan SR, Alarcon-Riquelme M, Bowie AG, Försti A, Weber AN, A coding IRAK2 protein variant compromises Toll-like receptor (TLR) signaling and is associated with colorectal cancer survival., The Journal of biological chemistry, 289, (33), 2014, p23123-31
Bowie AG, Rad50 and CARD9, missing links in cytosolic DNA-stimulated inflammation., Nature immunology, 15, (6), 2014, p534-6
Connolly DJ, Bowie AG, The emerging role of human PYHIN proteins in innate immunity: Implications for health and disease., Biochemical pharmacology, 2014
Gürtler C, Carty M, Kearney J, Schattgen SA, Ding A, Fitzgerald KA, Bowie AG, SARM regulates CCL5 production in macrophages by promoting the recruitment of transcription factors and RNA polymerase II to the Ccl5 promoter., Journal of immunology (Baltimore, Md. : 1950), 192, (10), 2014, p4821-32
Brady, Gareth Bowie, Andrew G., Innate immune activation of NFκB and its antagonism by poxviruses, Cytokine & Growth Factor Reviews, 2014
Carty M, Reinert L, Paludan SR, Bowie AG, Innate antiviral signalling in the central nervous system., Trends in immunology, 35, (2), 2014, 79-87
Paludan, S., Bowie, A., Immune Sensing of DNA, Immunity , 38, (5), 2013, p870-880
Paludan, S.R., Bowie, A.G., Horan, K.A., Fitzgerald, K.A., Recognition of herpesviruses by the innate immune system, Nature Reviews Immunology, 13, 2013, p614
O'Neill, L.A.J., Golenbock, D., Bowie, A.G., The history of Toll-like receptors-redefining innate immunity, Nature Reviews Immunology, 13, (6), 2013, p453-460
Bowie AG, Removing the TREX1 safety net: oxidized DNA overcomes immune silencing by exonuclease TREX1., Immunity, 39, (3), 2013, p423-5
Horan KA, Hansen K, Jakobsen MR, Holm CK, Søby S, Unterholzner L, Thompson M, West JA, Iversen MB, Rasmussen SB, Ellermann-Eriksen S, Kurt-Jones E, Landolfo S, Damania B, Melchjorsen J, Bowie AG, Fitzgerald KA, Paludan SR, Proteasomal degradation of herpes simplex virus capsids in macrophages releases DNA to the cytosol for recognition by DNA sensors., Journal of immunology (Baltimore, Md. : 1950), 190, (5), 2013, p2311-9
Stack J, Hurst TP, Flannery SM, Brennan K, Rupp S, Oda S, Khan AR, Bowie AG, Poxviral protein A52 stimulates p38 mitogen-activated protein kinase (MAPK) activation by causing tumor necrosis factor receptor-associated factor 6 (TRAF6) self-association leading to transforming growth factor ß-activated kinase 1 (TAK1) recruitment., The Journal of biological chemistry, 288, (47), 2013, p33642-53
Mansur DS, Maluquer de Motes C, Unterholzner L, Sumner RP, Ferguson BJ, Ren H, Strnadova P, Bowie AG, Smith GL, Poxvirus Targeting of E3 Ligase ß-TrCP by Molecular Mimicry: A Mechanism to Inhibit NF-"B Activation and Promote Immune Evasion and Virulence., PLoS pathogens, 9, (2), 2013, pe1003183
Gürtler, C., Bowie, A.G., Innate immune detection of microbial nucleic acids, Trends in Microbiology, 21, (8), 2013, p413-420
Stack J, Bowie AG, Poxviral protein A46 antagonizes Toll-like receptor 4 signaling by targeting BB loop motifs in Toll-IL-1 receptor adaptor proteins to disrupt receptor:adaptor interactions., The Journal of biological chemistry, 287, (27), 2012, p22672-22682
Bowie, A.G., Innate sensing of bacterial cyclic dinucleotides: More than just STING, Nature Immunology, 13, (12), 2012, p1137-1139
Bowie, A.G., The STING in the Tail for Cytosolic DNA-Dependent Activation of IRF3, Science Signalling, 5, 2012, ppe5
Pichlmair A, Kandasamy K, Alvisi G, Mulhern O, Sacco R, Habjan M, Binder M, Stefanovic A, Eberle CA, Goncalves A, Bürckstümmer T, Müller AC, Fauster A, Holze C, Lindsten K, Goodbourn S, Kochs G, Weber F, Bartenschlager R, Bowie AG, Bennett KL, Colinge J, Superti-Furga G, Viral immune modulators perturb the human molecular network by common and unique strategies., Nature, 487, (7408), 2012, p486-490
Leow-Dyke S, Allen C, Denes A, Nilsson O, Maysami S, Bowie AG, Rothwell NJ, Pinteaux E, Neuronal toll-like receptor 4 signaling induces brain endothelial activation and neutrophil transmigration in vitro., Journal of neuroinflammation, 9, 2012, p230
Tanveer R, Gowran A, Noonan J, Keating SE, Bowie AG, Campbell VA, The endocannabinoid, anandamide, augments Notch-1 signaling in cultured cortical neurons exposed to amyloid-ß and in the cortex of aged rats., The Journal of biological chemistry, 287, (41), 2012, p34709-21
Jin T, Perry A, Jiang J, Smith P, Curry JA, Unterholzner L, Jiang Z, Horvath G, Rathinam VA, Johnstone RW, Hornung V, Latz E, Bowie AG, Fitzgerald KA, Xiao TS, Structures of the HIN domain:DNA complexes reveal ligand binding and activation mechanisms of the AIM2 inflammasome and IFI16 receptor., Immunity, 36, (4), 2012, p561-571
Leonie Unterholzner, Rebecca P. Sumner, Marcin Baran, Hongwei Ren, Daniel S. Mansur, Nollaig M. Bourke, Felix Randow, Geoffrey L. Smith, Andrew G. Bowie, Vaccinia Virus Protein C6 Is a Virulence Factor that Binds TBK-1 Adaptor Proteins and Inhibits Activation of IRF3 and IRF7, PLoS Pathogens, 7, (9, e1002247), 2011
Paludan, S.R., Bowie, A.G., Horan, K.A., Fitzgerald, K.A., Recognition of herpesviruses by the innate immune system, Nature Reviews Immunology, 11, 2011, p143 - 154
Sinead M. Flannery, Sinead E. Keating, Joanna Syzmak and Andrew G. Bowie, HUMAN INTERLEUKIN-1 RECEPTOR ASSOCIATED KINASE-2 (IRAK-2) IS ESSENTIAL FOR TOLL-LIKE RECEPTOR-MEDIATED TRANSCRIPTIONAL AND POSTTRANSCRIPTIONAL REGULATION OF TNFα, Journal of Biological Chemistry, 286, 2011, p23688 - 23697
Unterholzner, L. & Bowie, A.G., Innate DNA Sensing Moves to the Nucleus, Cell Host and Microbe, 9, 2011, p351 - 353
Flannery, S.M. and Keating, S.E. and Szymak, J. and Bowie, A.G., Human interleukin-1 receptor-associated kinase-2 is essential for toll-like receptor-mediated transcriptional and post-transcriptional regulation of tumor necrosis factor α, Journal of Biological Chemistry, 286, (27), 2011, p23688-23697
Michael Carty, Andrew G. Bowie, Evaluating the role of Toll-like Receptors in diseases of the Central Nervous system, Biochemical Pharmacology, 81, (7), 2011, p825-837
Keating, S.E., Baran, M & Bowie, A.G., Cytosolic DNA sensors regulating type I interferon induction, Trends in Immunology, 32, 2011, p574 - 581
Paludan, S.R., Levy, O. & Bowie, A.G., Toll2011 at Lago di Garda: studying danger sensors by the guard towers at the lake, Nature Immunology, 12, 2011, p805 - 808
O'Neill LA, Bowie AG, The powerstroke and camshaft of the RIG-I antiviral RNA detection machine., Cell, 147, (2), 2011, p259-261
Brennan, K., Bowie, A.G., Activation of host pattern recognition receptors by viruses, Current Opinion in Microbiology, 13, (4), 2010, p503-507
Bowie, A.G, TRAF3: Uncovering the Real but Restricted Role in Human, Immunity, 33, (3), 2010, p293-295
Unterholzner, L., Keating, S.E., Baran, M., Horan, K.A., Jensen, S.B., Sharma, S., Sirois, C.M., (...), Bowie, A.G., IFI16 is an innate immune sensor for intracellular DNA, Nature Immunology, 11, 2010, p997-1004
Sinead Flannery and Andrew G. Bowie, The interleukin-1 receptor-associated kinases: critical regulators of innate immune signalling, Biochemical Pharmacology, 80, (12), 2010, p1981-1991
Lysakova-Devine, T., Keogh, B., Harrington, B., Nagpal, K., Halle, A., Golenbock, D.T., Monie, T. & Bowie, A.G. , Viral Inhibitory Peptide of TLR4, a Peptide Derived from Vaccinia Protein A46, Specifically Inhibits TLR4 by Directly Targeting MyD88 Adaptor-Like and TRIF-Related Adaptor Molecule, The Journal of Immunology, 185, 2010, p4261 - 4271
Toll-like receptor 3 in, editor(s)Hansel T.T., Barnes P.J. , Progress in Respiratory Research, Karger, 2010, pp73-79 , [Carty, M., Bowie, A.]
Carty M, Bowie AG, Recent insights into the role of Toll-like receptors in viral infection., Clinical and experimental immunology, 161, (3), 2010, p397-406
Carty, M., Bowie, A.G, Activation of host pattern recognition receptors by viruses, Clinical and Experimental Immunology, 161, (3), 2010, p397-406
O'Neill, L.A.J., Bowie, A.G., Sensing and Signaling in Antiviral Innate Immunity , Current Biology , 20, (7), 2010, pR328-R333
Mulhern, O., Bowie, A.G., Unexpected roles for DEAD-box protein 3 in viral RNA sensing pathways , European Journal of Immunology, 40, (4), 2010, p933-935
Keating, SE, Bowie, AG, The role of non-degradative ubiquitination in interleukin-1 and Toll-like receptor signalling., The Journal of Biological Chemistry, 284, (13), 2009, p8211 - 8215
Kalverda AP, Thompson GS, Vogel A, Schröder M, Bowie AG, Khan AR & Homans SW, Poxvirus K7 protein adopts a Bcl-2 fold: Biochemical mapping of its interactions with human DEAD box RNA helicase DDX3., Journal of Molecular Biology, 385, (3), 2009, p843-853
Characterisation of viral proteins that inhibit TLR signal transduction in, editor(s)O'Neill, L.A.J and McCoy, C. , Toll-Like Receptors, Methods of Molecular Biology, Humana Press, 2009, [Stack, J & Bowie, A.G.]
Role of Toll-Like Receptors in the Innate Immune Response to RNA Viruses in, editor(s)Brasier, A.R. et al. , Cellular Signaling and Innate Immune Responses to RNA Virus Infections, ASM Press, 2009, [Keating, S.E. & Bowie A.G.]
Uncovering novel gene function in TLR signalling using siRNA in, editor(s)O'Neill, L.A.J and McCoy, C. , Toll-Like Receptors, Methods of Molecular Biology, Humana Press, 2009, [Carty, M., Keating, S. & Bowie, A.G. ]
Bowie, A.G., TRIM-ing down Tolls, Nature Immunology, 9, (4), 2008, p348 - 350
Schröder, M, Baran, M, Bowie, AG, Viral targeting of DEAD box protein 3 reveals its role in TBK1/IKKepsilon-mediated IRF activation., The EMBO journal, 27, (15), 2008, p2147 - 2157
Hurst, T & Bowie, A.G, Innate immune signalling pathways: lessons from vaccinia virus, Future Virology, 3, (2), 2008, p147 - 156
Bowie, AG, Unterholzner, L, Viral evasion and subversion of pattern-recognition receptor signalling., Nature Reviews Immunology, 8, (12), 2008, p911 - 922
Modulation of Innate Immune Signalling Pathways by Viral Proteins in, editor(s)Fallon, P. , Pathogen-Derived Immunomodulatory Molecules, 2008, [Mulhern, O., Harrington, B. & Bowie, A.G.]
Toll-like receptor 3 in, New Drugs and Targets for Respiratory Diseases, Prous Science, 2008, [Carty, M. & Bowie, A.G. ]
Unterholzner, L. & Bowie, A.G. , The interplay between viruses and innate immune signalling: recent insights and therapeutic opportunities, Biochemical Pharmacology, 75, (3), 2008, p589 - 602
Bowie, A.G. & Fitzgerald, K.F. , RIG-I; Tri-ing to discriminate between self and non-self RNA, Trends in Immunology, 28, (4), 2007, p147 - 150
Innate Immune Signalling Pathways and Viral Recognition: New Perspectives in, editor(s)Gavin McAlpine , Focus on Signal Transduction Research, Nova Science Publishers, 2007, pp173 - 188, [Ismar R Haga & Andrew G Bowie]
Bowie, A.G., Recent advances in understanding the role of Toll-like receptors in anti-viral immunity, Clinical and Experimental Immunology, 147, (2), 2007, p217 - 226
O'Neill LA and Bowie AG, The family of five: TIR domain containing adapters in TLR signalling, Nat Rev Immunol, 7, 2007, p353-364
Marshall-Clarke,S., Downes, J.E., Haga, I.R., Bowie, A.G., Borrow, P., Pennock, J.L. Grencis, R.K. & Rothwell, P, Polyinosic acid is a ligand for Toll-like receptor 3, Journal of Biological Chemistry, 282, (34), 2007, p24759 - 24766
Keating, S.E., Maloney, G.M., Moran, E.M. & Bowie, A.G, IRAK-2 participates in multiple toll-like receptor signalling pathways to NFkappaB via activation of TRAF6 ubiquitination, The Journal of Biological Chemistry, 282, (46), 2007, p33435 - 33443
Andrew Bowie, Luke O'Neil, Julianne Stack, Geoff Smith, Ismar Haga, 'Orthopoxvirus vectors, genes and products thereof (A46R protein)', EPO, WO2004/031225, 2007
Andrew Bowie, Michael Carry, Martina Schroeder, Paul Moynagh, 'Compositions and methods for modulating an immune response (SARM)', IPO, WO 2007/096854, 2006
Boughan, P.K., Argent, R.H., Body-Malapel, M., Park, J-H., Ewings, K.E., Bowie, A.G., Ong, S.J., Cook, S.J., Sorensen, O.E., Manzo, B.A., Klein, N.J. Nuñez, G., Atherton, J.C. & Bajaj-Elliott, M., Nucleotide-binding oligomerisation domain-1 (NOD-1) and epidermal growth factor receptor (EGFR): critical regulators of Ò-defensins during Helicobacter Pylori infection, The Journal of Biological Chemistry, 281, (17), 2006, p11637 - 11648
Carty, M., Goodbody, R., Schröder, M., Stack, J., Moynagh, P.N. & Bowie, A.G. , The human adaptor SARM negatively regulates adaptor protein TRIF-dependent Toll-like receptor signalling, Nature Immunology, 7, (10), 2006, p1074 - 1081
Andrew Bowie, Martina Schroeder, Geoff Smith, Stuart Lucas, 'Compositions and methods for modulating an immune response (K7 protein)', EPO, WO 2008/023077 , 2006
Haga, I.H. and Bowie, A.G., Evasion of Innate Immunity by Vaccinia Virus, Parasitology, 130, 2005, pS11 - S25
Stack, J. Haga, I.R., Schroeder, M., Bartlett, N.W., Maloney, G., Reading, P.C., Fitzgerald, K.A., Smith, G.L. & Bowie, A.G, Vaccinia virus protein A46R targets multiple Toll-like-interleukin-1 receptor adaptors and contributes to virulence, The Journal of Experimental Medicine, 201, (6), 2005, p1007 - 1018
Bowie, A.G. & Haga, I.R. , The role of Toll like receptors in the host response to viruses. , Molecular Immunology, 42, 2005, p859 - 867
O'Toole D, Abde;-Latif AM, Long A, Windle HJ, Murphy AM, Bowie A, O'Neill LA, Weir DG, Kelleher D, Low pH and Helicobacter pylori increase nuclear factor kappa B binding in gastric epithelial cells: a common pathway for epithelial cell injury?, Journal of Cellular Biochemistry, 96, (3), 2005, p589 - 588
Toll-like receptors as key sensors of viral infection in, editor(s)L.A.J. O'Neill and E. Brint , Toll-like Receptors in Inflammation, 2005, pp143 - 171, [Keating, S.E. and Bowie, A.G.]
Schroeder, M & Bowie AG, TLR3 in antiviral immunity: key player or bystander?, Trends in Immunology, 26, (9), 2005, p462 - 468
Carroll, T.P., Greene, C.M., Taggart, C.C., Bowie, A.G., O'Neill, S.J. & McElvaney, N.G., Viral inhibition of IL-1 and neutrophil elastase-induced inflammatory responses in bronchial epithelial cells, The Journal of Immunology, 175, (11), 2005, p7594 - 7601
Maloney, G, Schroeder, M & Bowie, AG, Vaccinia virus protein A52R activates p38 mitogen-activated protein kinase and potentiates lipopolysaccharide-induced interleukin-10, The Journal of Biological Chemistry, 280, (280), 2005, p30838 - 30844
Melchjorsen, J, Jensen, S.B., Malmgaard,L., Rasmussen, S.B., Weber, F., Bowie, A.G., Matikainen, S. & Paludan, S.R. , Activation of Innate Defense against a Paramyxovirus Is Mediated by RIG-I and TLR7 and TLR8 in a Cell-Type-Specific Manner, The Journal of Virology, 79, (20), 2005, p12944 - 12951
Brady, G, Boggan, L, Bowie, AG & O'Neill, LAJ, Schlafen-1 causes cell cycle arrest by inhibiting induction of cyclin D1, The Journal of Biological Chemistry, 280, 2005, p30723 - 30734
Malmgaard, L., Melchjorsen, J., Bowie, A.G., Mogensen, S.C. & Paludan, S.R. , Viral activation of macrophages through TLR-dependent and -independent pathways., The Journal of Immunology, 173, (11), 2004, p6890 - 6898
Bowie, A.G., Zhan, J. & Marshall, W.L., Viral Appropriation of Apoptotic and NF-kappaB Signalling Pathways, Journal of Cell Biology, 91, (6), 2004, p1099 - 1108
DiPerna, G., Stack, J., Bowie, A.G., Boyd, A., Kotwal, G., Zhang, Z., Arvikar, S., Latz, E., Fitzgerald, K.A. & Marshall, W.L. , Poxvirus protein N1L targets the I-kappaB Kinase complex, inhibits signaling to NF-kappaB by the Tumor Necrosis Factor superfamily of receptors, and inhibits NF-kappaB and IRF3 signaling by Toll-like receptors. , The Journal of Biological Chemistry, 279, (35), 2004, p36570 - 36578
L. A. O'Neill, K. A. Fitzgerald and A. G. Bowie, The Toll-IL-1 receptor adaptor family grows to five members, Trends in immunology, 24, (6), 2003, p286-90
Modulation of Toll-like receptor signalling by viruses in, editor(s)C.D O'Connor and D.G.E Smith , SGM symposium 62: Microbial subversion of host cells, Cambridge, Cambridge University Press, 2003, pp109 - 128, [Bowie, A. ]
Harte, MT, Haga, I, Maloney, G, Gray, P, Reading, PC, Bartlett, N, Smith, GL, Bowie, AG and O'Neill, LAJ , The poxvirus protein A52R targets Toll-like receptor signaling complexes to suppress host defence, Journal of Experimental Medicine, 197, (3), 2003, p343 - 351
Andrew Bowie, Luke O'Neill, Mary Harte, Geoff Smith, Ismar Haga, Geraldine Maloney , 'Orthopoxvirus vectors, genes and products thereof (A52R protein). ', EPO, WO 2004/022762, 2003
K. A. Fitzgerald, E. M. Palsson-McDermott, A. G. Bowie, C. A. Jefferies, A. S. Mansell, G. Brady, E. Brint, A. Dunne, P. Gray, M. T. Harte, D. McMurray, D. E. Smith, J. E. Sims, T. A. Bird and L. A. O'Neill, Mal (MyD88-adapter-like) is required for Toll-like receptor-4 signal transduction, Nature, 413, (6851), 2001, p78-83
O'Neill, LAJ and Bowie, A (eds.), Interleukin protocols, New Jersey, The Humana Press Inc, 2001
C. Jefferies, A. Bowie, G. Brady, E. L. Cooke, X. Li and L. A. O'Neill, Transactivation by the p65 subunit of NF-kappaB in response to interleukin-1 (IL-1) involves MyD88, IL-1 receptor-associated kinase 1, TRAF-6, and Rac1, Molecular and Cellular Biology, 21, (14), 2001, p4544-4552
A. Bowie and L. A. O'Neill, The interleukin-1 receptor/Toll-like receptor superfamily: signal generators for pro-inflammatory interleukins and microbial products, Journal of Leukocyte Biology, 67, (4), 2000, p508-514
A. Bowie and L. A. O'Neill, Oxidative stress and nuclear factor-kappaB activation: a reassessment of the evidence in the light of recent discoveries, Biochemical pharmacology, 59, (1), 2000, p13-23
A. G. Bowie and L. A. O'Neill, Vitamin C inhibits NF-kappa B activation by TNF via the activation of p38 mitogen-activated protein kinase, Journal of Immunology, 165, (12), 2000, p7180-7188
K. A. Fitzgerald, A. G. Bowie, B. S. Skeffington and L. A. O'Neill, Ras, protein kinase C zeta, and I kappa B kinases 1 and 2 are downstream effectors of CD44 during the activation of NF-kappa B by hyaluronic acid fragments in T-24 carcinoma cells, Journal of Immunology , 164, (4), 2000, p2053-2063
A. Bowie, E. Kiss-Toth, J. A. Symons, G. L. Smith, S. K. Dower and L. A. O'Neill, A46R and A52R from vaccinia virus are antagonists of host IL-1 and toll-like receptor signaling, Proceedings of the National Academy of Sciences of the United States of America, 97, (18), 2000, p10162-10167
McGowan AJ, Bowie AG, O'Neill LA, Cotter TG., The production of a reactive oxygen intermediate during the induction of apoptosis by cytotoxic insult., Experimental Cell Research, 238, (1), 1998, p248 - 256
A. G. Bowie, P. N. Moynagh and L. A. O'Neill, Lipid peroxidation is involved in the activation of NF-kappaB by tumor necrosis factor but not interleukin-1 in the human endothelial cell line ECV304. Lack of involvement of H2O2 in NF-kappaB activation by either cytokine in both primary and transformed endothelial cells, The Journal of biological chemistry, 272, (41), 1997, p25941-25950
Bowie, A., Owens, D., Collins, P., Johnson, A. and Tomkin, G.H. , Glycosylated low density lipoprotein is more sensitive to oxidation: implications for the diabetic patient? , Atherosclerosis , 102, (1), 1993, p63 - 67
Non-Peer-Reviewed Publications
Bowie, A.G., Insights from vaccinia virus into Toll-like receptor signalling proteins and their regulation by ubiquitin: role of IRAK-2, Biochemistry Society Transactions, 36, (3), 2008, pp449-452
M. Schröder and A. G. Bowie, An arms race: Innate anti-viral responses and counter-acting viral strategies., Biochemistry Society Transactions, Biochemistry Society Transactions, 35, (6), 2007, pp1512-1514
Bowie, A. and O'Neill, L.A.J., Vitamin C inhibits NF kappa B activation in endothelial cells., Biochemistry Society Transactions, 25, (1), 1997, pp131S.
Bowie, A. and O'Neill, L.A.J., Studies into the mechanism of NF kappa B activation by IL1, TNF and H2O2 in primary and transformed endothelial cells., Biochemistry Society Transactions, 25, (1), 1997, pp125S
A. Bowie, P. N. Moynagh and L. A. O'Neill, Mechanism of NF kappa B activation by interleukin-1 and tumour necrosis factor in endothelial cells, 24, (1), 1996, pp2S
A. Bowie, P. N. Moynagh and L. A. O'Neill, The human endothelial cell line ECY304 as a model of endothelial cell activation by interleukin-1, 23, (1), 1995, pp109S
Brennan, P., Bowie, A. and O'Neill, L.A.J., The Effect of Thiol Modifiers on the Activation of NF kappa B by Interleukin-1., 21, (4), 1993, pp390S
Research Expertise
Description
My research focuses on innate immune sensing and signalling mechanisms, and their modulation by viruses. Specifically we are looking at how viruses interact with, and seek to subvert innate immune signalling pathways, such Toll-like receptors (TLRs) and RIG-I-like receptors (RLRs). TLRs and RLRs represent host immune detection systems, in that they have been shown to be involved in recognising pathogens and activating the innate immune response. Through both bioinformatics and functional screens we have identified many poxviral proteins which can antagonise innate immune signalling, and current on-going investigations are seeking to understand the mechanism of action of these proteins and to identify and characterise host target proteins. Further, peptides derived from inhibitory viral proteins are being tested as a route to developing novel anti-inflammatory drugs. Work in my laboratory has shed light on how pattern recognition receptors (PRRs) recognise pathogens, leading to the induction of interferons and cytokines, which control infection locally as well as coordinating the adaptive immune response. We also investigate how PRRs and other innate immune sensors drive inflammation through the recognition of nucleic acid such as mislocalised self-DNA. I have recently identified novel roles for a number of innate immune proteins in regulating inflammation via cytokine modulation, and am currently exploring their mechanisms of actionRecognition
Awards and Honours
Highly cited researcher (Clarivate, top 1% by citations)
Irish Society for Immunology Public Lecture Award Medal
Elected to the Royal Irish Academy
Elected Fellow of TCD
Sheldon Wolff Prize in Cytokine Research
Placed first in class (B.A. (Mod)).
Memberships
Royal Irish Academy
The International Cytokine and Interferon Society
American Society for Biochemistry and Molecular Biology
American Society for Microbiology