Gallery
Cellular internalization of uncoated gold nanoboxes (AuNBs) in mono-cultures of A549 cells after 24 h exposure. Projections and rendered reconstructions of representative confocal micrographs of A549 cells exposed to AuNBs and stained with rhodamine phalloidin (F-actin, in red) and Hoechst 33342 (nuclei, in blue). AuNBs were imaged in confocal reflectance mode and are shown in green as pseudo-colour. The localization of AuNBs (indicated by arrows) in the cells cytoplasm is evident. Scale bars: 10 mm (63x objective lens).
Cellular internalization of gold nanoboxes (AuNBs) in 3D cell cultures grown onto PuraMatrix (0.25%). TEM images of ultrathin cross-sections (80 nm) of 3D cell cultures exposed to AuNB2 for 24 h AuNBs (indicated by white arrows) can be recognized by their geometrical shape and contrast as internalized in lysosomes and lamellar bodies. Images ii-v are magnifications of image i. Abbreviations: lb: lamellar bodies; ls: lysosome; n: nucleus.
Rendering of representative confocal images of gold nanoboxes (AuNBs)-treated cells, stained with rhodamine phalloidin (F-actin, in red) and Hoechst 33342 (nuclei, in blue). AuNBs (indicated by arrows) were imaged in confocal reflectance mode and are shown in green as pseudo-colour. (ii) Detail of image i. The light-blue rectangle represents the clipping plane. The presence of a single AuNB inside the cell cytoplasm is highlighted. Scale bars: (i) 10 mm, (ii) 5 mm (63x objective lens).
Source:Dania Movia, Valerie Gerard, Ciaran Manus Maguire, Namrata Jain, Alan P. Bell, Valeria Nicolosi, Tina ONeill, Dimitri Scholz , Yuri Gunko, Yuri Volkov, Adriele Prina-Mello., Biomaterials (2014).
Coloured HIM image of gold nanoboxes (AuNBs) (in yellow) after 24 h incubation with A549 cells. The AuNBs coating with biological material is evident. Scale bar: 100 nm.
Source:Dania Movia, Valerie Gerard, Ciaran Manus Maguire, Namrata Jain, Alan P. Bell, Valeria Nicolosi, Tina ONeill, Dimitri Scholz , Yuri Gunko, Yuri Volkov, Adriele Prina-Mello., Biomaterials (2014).
(i) Representative TEM images and (ii) HIM images of flat-lying and edge-oriented uncoated gold nanoboxes (AuNBs) of different sizes (AuNB1 = and AuNB2 = ) before (0 h) and after incubation with A549 cells for 24, 48 and 72 h. Arrows indicate distinct signs of AuNBs degradation. Scale bars: (i) 50 nm; (ii) 100 nm.
Representative TEM images of ultrathin cross-sections (80 nm) of A549 cells exposed to gold nanoboxes for 24 h. Gold nanoboxes (indicated by arrows) can be recognized by their geometrical shape and contrast as internalized in vacuoles and lysosomes. Images iii and iv are magnifications of images i and ii, respectively. Abbreviations: er, endoplasmic reticulum; ls: lysosome; m: microvilli; mt: mitochondria; n: nucleus; vc: vacuole.