Professor Conor Buckley
Professor in, Mechanical, Manuf & Biomedical Eng
Biography
Conor Buckley is Professor In Biomedical Engineering and Director of the Trinity Centre for Biomedical Engineering. Buckley is a principal investigator (PI) in both the Trinity Centre for Biomedical Engineering and Science Foundation Ireland (SFI) national centre for Advanced Materials and Bioengineering Research (AMBER) Centre at Trinity College Dublin and Honorary Associate Professor in the Royal College of Surgeons in Ireland. His research focuses on novel biomaterials, biofabrication and cell-based strategies for tissue regeneration
Buckley has published over 90 international peer reviewed journal articles and over 200 conference publications. Buckley is a reviewer for 29 leading journals in the fields of Biomaterials and Tissue Engineering and serves on the International Advisory Review Board (ARB) for the Journal of Orthopaedic Research-Spine.
Buckley is also a PI on several commercial projects developing biomaterials for peripheral nerve repair and bioprinting for next generation implantable devices and tissues for orthopaedic applications. He also has experience in the commercialisation of technology including the filing and licensing of patents in relation to porous biomaterials for nerve (PCT/EP2020/083811) and cartilage tissue regeneration (EP18215447.6). In 2022, he co-founded Altach, (https://altach.health/) a campus spin-out company pioneering off-the-shelf cartilage scaffolds to regenerate damaged knee joints.
Prof. Buckley's laboratory has been recognised through notable honours and distinctions including the Irish Research Council (IRC) New Foundations Award, Engineers Ireland Biomedical Research Medal, European Society of Biomechanics S.M. Perren Award. He is a recipient of a Science Foundation Ireland Career Development Award (CDA, 2016), a European Research Council (ERC) Consolidator award to develop personalised medicine approaches to regenerate the intervertebral disc (2019) and an ERC Proof of Concept award for injectable biomimetic self-healing tissue adhesives (2024).
Current research activities: Personalised medicine and injectable gene activated biomaterials for intervertebral disc repair; Biofabrication of electroconductive ECM nerve guidance conduits; Injectable biomimetic self-healing tissue adhesives; 3D printing of ECM derived orthopaedic models and implants; Predictive in silico metabolite modelling for cell-based therapies.
Publications and Further Research Outputs
Peer-Reviewed Publications
McDonnell E.E., Ni Neill T., Wilson N., Darwish S.L., Butler J.S., Buckley C.T., In silico modeling the potential clinical effect of growth factor treatment on the metabolism of human nucleus pulposus cells, JOR Spine, 7, (3), 2024
Salinas-Fernandez, S. and Garcia, O. and Kelly, D.J. and Buckley, C.T., The influence of pH and salt concentration on the microstructure and mechanical properties of meniscus extracellular matrix-derived implants, Journal of Biomedical Materials Research - Part A, 112, (3), 2024, p359-372
Barcellona, M.N. and Ní Néill, T. and O'Brien, F.J. and Dixon, J.E. and Curtin, C.M. and Buckley, C.T., Modulation of Inflammation and Regeneration in the Intervertebral Disc Using Enhanced Cell-Penetrating Peptides for MicroRNA Delivery, Advanced NanoBiomed Research, 2024
Gansau, J. and McDonnell, E.E. and Buckley, C.T., Development and characterization of antacid microcapsules to buffer the acidic intervertebral disc microenvironment, Journal of Biomedical Materials Research - Part A, 2024
Basatvat S., Bach F.C., Barcellona M.N., Binch A.L., Buckley C.T., Bueno B., Chahine N.O., Chee A., Creemers L.B., Dudli S., Fearing B., Ferguson S.J., Gansau J., Gantenbein B., Gawri R., Glaeser J.D., Grad S., Guerrero J., Haglund L., Hernandez P.A., Hoyland J.A., Huang C., Iatridis J.C., Illien-Junger S., Jing L., Kraus P., Laagland L.T., Lang G., Leung V., Li Z., Lufkin T., van Maanen J.C., McDonnell E.E., Panebianco C.J., Presciutti S.M., Rao S., Richardson S.M., Romereim S., Schmitz T.C., Schol J., Setton L., Sheyn D., Snuggs J.W., Sun Y., Tan X., Tryfonidou M.A., Vo N., Wang D., Williams B., Williams R., Yoon S.T., Le Maitre C.L., Harmonization and standardization of nucleus pulposus cell extraction and culture methods, JOR Spine, 6, (1), 2023
McDonnell E.E., Wilson N., Barcellona M.N., Ni Neill T., Bagnall J., Brama P.A.J., Cunniffe G.M., Darwish S.L., Butler J.S., Buckley C.T., Preclinical to clinical translation for intervertebral disc repair: Effects of species-specific scale, metabolism, and matrix synthesis rates on cell-based regeneration, JOR Spine, 2023
Samuel, S. and McDonnell, E.E. and Buckley, C.T., Effects of Growth Factor Combinations TGFβ3, GDF5 and GDF6 on the Matrix Synthesis of Nucleus Pulposus and Nasoseptal Chondrocyte Self-Assembled Microtissues, Applied Sciences (Switzerland), 12, (3), 2022
Barcellona M.N., McDonnell E.E., Samuel S., Buckley C.T., Rat tail models for the assessment of injectable nucleus pulposus regeneration strategies, JOR Spine, 2022
McDonnell, E.E. and Buckley, C.T., Consolidating and re-evaluating the human disc nutrient microenvironment, JOR Spine, 5, (1), 2022
Hibbitts, A.J. and KoÄ Ã, Z. and Kneafsey, S. and Matsiko, A. and ÅœiliÄ , L. and Dervan, A. and Hinton, P. and Chen, G. and Cavanagh, B. and Dowling, J.K. and McCoy, C.E. and Buckley, C.T. and Archibald, S.J. and O'Brien, F.J., Multi-factorial nerve guidance conduit engineering improves outcomes in inflammation, angiogenesis and large defect nerve repair, Matrix Biology, 106, 2022, p34-57
Browe D.C., Burdis R., Diaz-Payno P.J., Freeman F.E., Nulty J.M., Buckley C.T., Brama P.A.J., Kelly D.J., Promoting endogenous articular cartilage regeneration using extracellular matrix scaffolds, Materials Today Bio, 16, 2022
Browe, D.C. and DÃaz-Payno, P.J. and Freeman, F.E. and Schipani, R. and Burdis, R. and Ahern, D.P. and Nulty, J.M. and Guler, S. and Randall, L.D. and Buckley, C.T. and Brama, P.A.J. and Kelly, D.J., Bilayered extracellular matrix derived scaffolds with anisotropic pore architecture guide tissue organization during osteochondral defect repair, Acta Biomaterialia, 143, 2022, p266-281
McDonnell E.E., Buckley C.T., Two- and three-dimensional in vitro nucleus pulposus cultures: An in silico analysis of local nutrient microenvironments, JOR Spine, 2022
McDonnell, Emily E., Buckley, Conor T., Investigating the physiological relevance of ex vivo disc organ culture nutrient microenvironments using in silico modeling and experimental validation, JOR SPINE, 2021
Gansau J, Buckley CT., Priming as a strategy to overcome detrimental pH effects on cells for intervertebral disc regeneration., European cells & materials, 41, 2021, p153-169
Carroll SF, Buckley CT, Kelly DJ., Measuring and Modeling Oxygen Transport and Consumption in 3D Hydrogels Containing Chondrocytes and Stem Cells of Different Tissue Origins., Frontiers in bioengineering and biotechnology, 9, 2021, p591126
Chiara Borrelli, Conor T. Buckley, Injectable Disc-Derived ECM Hydrogel Functionalised with Chondroitin Sulfate for Intervertebral Disc Regeneration, Acta Biomateralia, 117, 2020, p142 - 155
Chiara Borrelli, Conor T. Buckley, Synergistic Effects of Acidic pH and Pro-Inflammatory Cytokines IL-1ß and TNF-α for Cell-Based Intervertebral Disc Regeneration, Applied Sciences, 10, (24), 2020, p9009
Conor Buckley, Leyla Zilic, Fergal O'Brien , 'An acellular nerve graft', 0, 2019
Hibbitts A.J., Koci Z., Kneafsey S., Zilic L., Cavanagh B., Buckley C.T., Archibald S.J. and O'Brien F.J., Effect of matrix substrate composition on in vitro and in vivo regeneration in microporous nerve guidance conduits, Tissue Engineering and Regenerative Medicine International Society EU (TERMIS-EU), Rhodes, Greece, 2019
Conor Buckley, Engineering Biomaterial and Cell-based Strategies for Disc Regeneration- Layers of Complexity, ORS PSRS 5th International Spine Research Symposium- New Horizons in Intervertebral Disc Research, Skytop Lodge, Skytop, Pennsylvania, USA, Nov 3 - 7, 2019
Gansau J., McDonnell E. and Buckley C.T. , Altering the Local Acidic Microenvironment of the Degenerated Intervertebral Disc using Injectable Antacid Microcapsules, ORS PSRS 5th International Spine Research Symposium- New Horizons in Intervertebral Disc Research, Skytop Lodge, Skytop, Pennsylvania, USA, 2019
Browe D.C., Burdis R., Freeman F.E., Diaz Payno P.J., Nulty J.M., Buckley C.T., Brama P.A.J. and Kelly D.J. , Enhancing endogenous articular cartilage repair using extracellular matrix derived scaffolds: Evaluation in a caprine model, Tissue Engineering and Regenerative Medicine International Society EU (TERMIS-EU), Rhodes, Greece, 2019
Browe D., Burdis, R., Freeman, F.E., Diaz Payno, P., Nulty, J.M., Buckley C.T., Brama P.A.J. and Kelly D.J. , Enhancing Endogenous Articular Cartilage Repair Following Microfracture Using Superelastic Extracellular Matrix Derived Scaffolds, Orthopaedic Research Society, Austin Texas, USA, 2019
McDonnell E., Tornifoglio B., Gansau J. and Buckley C.T., Elucidating and Optimizing the Distribution of Metabolites within Ex Vivo Disc Organ Culture Systems, ORS PSRS 5th International Spine Research Symposium- New Horizons in Intervertebral Disc Research, Skytop Lodge, Skytop, Pennsylvania, USA, 2019
Naqvi, S.M. and Gansau, J. and Gibbons, D. and Buckley, C.T., In vitro co-culture and ex vivo organ culture assessment of primed and cryopreserved stromal cell microcapsules for intervertebral disc regeneration, European cells & materials, 37, 2019, p134-152
Kočí Z., Hibbits A.J., Kneafsey S.L., Zilic L., Chen G., Buckley C.T., Archibald S.J. and O'Brien F.J, An advanced nerve guidance conduit for repairing large peripheral nerve defects, Society for Neuroscience meeting, Neuroscience , McCormick Place, Chicago, Illinois, United States., 2019
Samuel S., McDonnell E., and Buckley C.T., Effect of Growth Factor Priming to Promote a Discogenic Phenotype of Tissue Spheroids for Intervertebral Disc Regeneration, ORS PSRS 5th International Spine Research Symposium- New Horizons in Intervertebral Disc Research, Skytop Lodge, Skytop, Pennsylvania, USA, 2019
Browe, D.C. and Mahon, O.R. and DÃaz-Payno, P.J. and Cassidy, N. and Dudurych, I. and Dunne, A. and Buckley, C.T. and Kelly, D.J., Glyoxal cross-linking of solubilized extracellular matrix to produce highly porous, elastic, and chondro-permissive scaffolds for orthopedic tissue engineering, Journal of Biomedical Materials Research - Part A, 2019
Reina Rodriguez F.S., Buckley C.T. and Kirby B.M., Biomechanical studies of feline ventral abdominal wall and celiotomy closure techniques., Veterinary Surgery, 47, (2), 2018, p193-203
Conor Buckley, Cell Priming to Sustain the Acidic Microenvironment of the Intervertebral Disc, ARI-TCBE symposium, RCSI, Dublin, Ireland, 28th September , 2018
Gansau, J. and Kelly, L. and Buckley, C.T., Influence of key processing parameters and seeding density effects of microencapsulated chondrocytes fabricated using electrohydrodynamic spraying, Biofabrication, 10, (3), 2018
Buckley C.T., Gansau J., Irwin P., and Borrelli C. , Optimizing Glucose Microenvironmental Conditions for Physiologically Relevant Culture of Bovine Disc Explants, 64th Annual Meeting of the Orthopaedic Research Society, Hyatt Regency, New Orleans, Louisiana, USA, 2018
Gansau J. and Buckley C.T. , The Effect of Priming on Matrix Accumulation and Metabolism of Stem Cells and Chondrocytes in Altered Intervertebral Disc-like pH Conditions, 26th EORS Annual Meeting, Galway, Ireland, 2018
Conor T. Buckley, Judith A. Hoyland, Kengo Fujii, Abhay Pandit, James C. Iatridis, Sibylle Grad, Critical aspects and challenges for intervertebral disc repair and regeneration-Harnessing advances in tissue engineering, JOR Spine, 1, (3), 2018, pe1029
Browe D., Buckley C.T. and Kelly D.J., Evaluation of Extracellular Matrices Derived from Various Tissue Sources to Modulate Mesenchymal Stromal Cell Differentiation for Musculoskeletal Tissue Engineering, 8th World Congress of Biomechanics, Dublin, Ireland, 2018
Conor Buckley, Regeneration of the IVD- Priming and conditioning of cells to function in the disc microenvironment, eCM XVIII: Cartilage & Disc: Repair and Regeneration, Congress Center, Davos, Switzerland, 25th - 28th June, 2018
Lachlan J. Smith, Lara Silverman, Daisuke Sakai, Christine L. Le Maitre, Robert L. Mauck, Neil R. Malhotra, Jeffrey C. Lotz, Conor T. Buckley, Advancing cell therapies for intervertebral disc regeneration from the lab to the clinic: Recommendations of the ORS spine section, JOR Spine, 1, (4), 2018, pe1036
Gansau J. and Buckley C.T. , Investigation of Various Cell Types for Intervertebral Disc Repair under Disc Degenerative Culture Conditions, 8th World Congress of Biomechanics, Dublin, Ireland, 2018
Borrelli C. and Buckley C.T. , Solubilised Extracellular Matrix Derived from Nucleus Pulposus Tissue, 8th World Congress of Biomechanics, Dublin, Ireland, 2018
Naqvi, S.M. and Gansau, J. and Buckley, C.T., Priming and cryopreservation of microencapsulated marrow stromal cells as a strategy for intervertebral disc regeneration, Biomedical Materials (Bristol), 13, (3), 2018
Gansau, J. and Buckley, C.T., Incorporation of collagen and hyaluronic acid to enhance the bioactivity of fibrin-based hydrogels for nucleus pulposus regeneration, Journal of Functional Biomaterials, 9, (3), 2018, p43-
Buckley, C. T., Engineering Injectable Cellular Microcapsules for the Intervertebral Disc Microenvironment Niche., ORS Musculoskeletal Summer Symposium, Dublin, Ireland, July 6th-7th, 2017
Vedicherla, S., Buckley, C.T., Cell-based therapies for intervertebral disc and cartilage regeneration- Current concepts, parallels, and perspectives, Journal of Orthopaedic Research, 35, (1), 2017, p8 - 22
Gansau J. and Buckley C.T., Injectable cell-loaded microcapsules as a potential strategy for nucleus pulposus regeneration., BioSpine 2017- 6th International Congress on Biotechnologies for Spinal Surgery, Langenbeck-Virchow-Haus, Berlin, Germany, 2017
Srujana Vedicherla, Conor Timothy Buckley, Rapid Chondrocyte Isolation for Tissue Engineering Applications: The Effect of Enzyme Concentration and Temporal Exposure on the Matrix Forming Capacity of Nasal Derived Chondrocytes, BioMed Research International, 2017, 2017, p2395138-
Vedicherla S.V., Withers D., Moran C.J. and Buckley C.T. , Enhanced Integration using Freshly Isolated Human Infrapatellar Fat Pad Cell and Chondrocyte Coculture with a Polyurethane Meniscal Replacement, 63rd Annual Meeting of the Orthopaedic Research Society, San Diego Convention Center, California, USA, 2017
Carroll, S.F., Buckley, C.T. and Kelly, D.J., Cyclic tensile strain can play a role in directing both intramembranous and endochondral ossification of mesenchymal stem cells., Frontiers in Bioengineering and Biotechnology, section Tissue Engineering and Regenerative Medicine. , 5, 2017, p73-
Buckley C.T. , Cells and Injectable Biomaterials - Engineering for the Disc Microenvironment, Orthopaedic Research Society Spine Section Meeting, San Diego Convention Center, USA, March 19th, 2017
Vedicherla, S. and Buckley, C.T., In vitro extracellular matrix accumulation of nasal and articular chondrocytes for intervertebral disc repair, Tissue and Cell, 49, (4), 2017, p503-513
Vedicherla S.V. and Buckley C.T. , In Vitro Extracellular Matrix Accumulation of Nasal and Articular Chondrocytes for Intervertebral Disc Repair, Tissue and Cell , 49, (4), 2017, p503-513
Buckley C.T. , Engineering solutions to regenerate-Injectable cellular microcapsules for intervertebral disc repair. Cell Matrix and Regenerative Medicince (CMRM) Seminar Series, Cell Matrix and Regenerative Medicince (CMRM) Seminar Series, Manchester University, UK, 9th June , 2017, Wellcome Trust Centre for Cell-Matrix Research and the Division of Cell Matrix Biology and Regenerative Medicine
Luo, L., O'Reilly, A.R., Thorpe, S.D., Buckley, C.T., Kelly, D.J., Engineering zonal cartilaginous tissue by modulating oxygen levels and mechanical cues through the depth of infrapatellar fat pad stem cell laden hydrogels, Journal of Tissue Engineering and Regenerative Medicine, 11, (9), 2017, p2613-2628
Gilroy, D.A. and Hobbs, C. and Nicolosi, V. and Buckley, C.T. and O'Brien, F.J. and Kearney, C.J., Development of magnetically active scaffolds as intrinsically-deformable bioreactors, MRS Communications, 7, (3), 2017, p367-374
Almeida, H.V. and Sathy, B.N. and Dudurych, I. and Buckley, C.T. and O'Brien, F.J. and Kelly, D.J., Anisotropic Shape-Memory Alginate Scaffolds Functionalized with Either Type I or Type II Collagen for Cartilage Tissue Engineering, Tissue engineering. Part A, 23, (1-2), 2017, p55-68
Vedicherla S.V., Romanazzo S., Kelly D.J., Buckley C.T.* and Moran C.J.* , Chondrocyte based Intraoperative Processing Strategies for the Biological Augmentation of a Polyurethane Meniscal Replacement, Connective Tissue Research , 59, (4), 2017, 381-392
Buckley C.T. , Cellular microencapsulation and microenvironmental challenges for intervertebral disc regeneration, AOSpine Masters Symposium-Novel and Emerging Technologies in Translational Medicine , Bern, Switzerland, 22 - 23 September , 2017
Browe D., Mahon O., Dudurych I., Dunne A., Buckley C.T. and Kelly D.J., The Combination of Rapidly Isolated Infrapatellar Fat Pad Stromal Cells and Glyoxal Cross-linked Type II Collagen Scaffolds As a Single-stage Therapy For Articular Cartilage Repair, 63rd Annual Meeting of the Orthopaedic Research Society, San Diego Convention Center, California, USA, 2017
Carroll S.F., Sathy B.N., Buckley C.T. and Kelly D.J. , Cyclic Tensile Strain Directs Both Intramembranous And Endochondral Ossification Of MSCs, 63rd Annual Meeting of the Orthopaedic Research Society, San Diego Convention Center, California, USA, 2017
Naqvi S.M., Vedicherla S.V., Gansau J, McIntyre T, Doherty M and Buckley C.T., Living Cell Factories- Electrosprayed Microcapsules and Microcarriers for Minimally Invasive Delivery, Advanced Materials , 28, (27), 2016, p5662-5671-
Naqvi, S.M. and Buckley, C.T. , Bone Marrow Stem Cells in Response to Intervertebral Disc-Like Matrix Acidity and Oxygen Concentration - Implications for Cell-Based Regenerative Therapy, Spine, 41, (9), 2016, p743 - 750
Browe D.C., Dudurych I., Buckley C.T. and Kelly D.J. , Combining glyoxal cross-linked type II collagen based scaffolds with infrapatellar fat pad stromal cells for articular cartilage repair, Tissue Engineering and Regenerative Medicine International Society EU (TERMIS-EU), Uppsala, Sweden, 2016
Almeida, H.V., Eswaramoorthy, R., Cunniffe, G.M., Buckley, C.T., O'Brien, F.J., Kelly, D.J., Fibrin hydrogels functionalized with cartilage extracellular matrix and incorporating freshly isolated stromal cells as an injectable for cartilage regeneration, Acta Biomaterialia, 36, 2016, p55-62
Gansau J. and Buckley C.T. , Effect of fibrin concentration on matrix deposition capacity of articular chondrocytes for nucleus pulposus regeneration, Tissue Engineering and Regenerative Medicine International Society EU (TERMIS-EU), Uppsala, Sweden, 2016
Carroll S.F., Sathy B.N., Buckley C.T. and Kelly D.J. , Cyclic tensile strain enhances both intramembranous and endochondral ossification of MSCs encapsulated within fibrin hydrogels, Tissue Engineering and Regenerative Medicine International Society EU (TERMIS-EU), Uppsala, Sweden, 2016
Carroll S.F., Sathy B.N., Buckley C.T. and Kelly D.J. , Mechanical cues and cell shape regulate the myogenic versus chondrogenic fate decision of MSCs, Tissue Engineering and Regenerative Medicine International Society EU (TERMIS-EU), Uppsala, Sweden, 2016
Mesallati T., Buckley C.T. and Kelly D.J. , Engineering cartilaginous grafts using chondrocyte-laden hydrogels supported by a superficial layer of stem cells, Journal of Tissue Engineering and Regenerative Medicine , 5, (18), 2016, p2353-2362
Naqvi S.M. and Buckley C.T. , Cryopreservation of Primed Microencapsulated Bone Marrow Derived Stem Cells Using Electrohydrodynamic Spraying For On Demand Minimally Invasive Tissue Regeneration of the Intervertebral Disc, 4th Tissue Engineering and Regenerative Medicine International Society (TERMIS) World Congress, Boston, USA., 2015
Almeida, A.V, Cunniffe, G.M., Vinardell, T., Buckley, C.T., O'Brien, F.J., Kelly, D.J. , Coupling freshly isolated CD44+ infrapatellar fat pad derived stromal cells with a tgf-β3 eluting cartilage ecm-derived scaffold as a single stage strategy for promoting chondrogenesis. , Advanced Healthcare Materials, 4, (7), 2015, 1043-1053
Naqvi S.M. and Buckley C.T. , Effect of Priming and Structured Co-culture of Microencapsulated Bone Marrow and Adipose Tissue Derived Stem Cells for Disc Repair, 61st Annual Meeting of the Orthopaedic Research Society, MGM Grand Hotel in Las Vegas, Nevada, USA, 2015
Guillaume, O., Naqvi, S.M., Lennon, K. and Buckley C.T. , Enhancing cell migration in shape-memory alginate-collagen composite scaffolds: in vitro and ex vivo assessment for intervertebral disc repair, Journal of Biomaterials Applications, 29, (9), 2015, p1230 - 1246
Buckley C.T. and Vedicherla S.V. , Towards a Single Stage Procedure for Intervertebral Disc Repair using Nasal Septal Chondrocytes, ORS PSRS 3rd International Spine Research Symposium, Philadelphia, USA, 2015
Sanders, R., Kearney, C, Buckley, C.T., Jenner, F. and Brama, P. , Knot security of 5 metric (USP 2) sutures: influence of knotting technique, suture material, and incubation time for 14 and 28 days in phosphate buffered saline and inflamed equine peritoneal fluid, Veterinary Surgery, 44, (6), 2015, p723 - 730
Almeida H.V, Cunniffe G.M, Vinardell T, Buckley C.T, O'Brien F.J, Kelly D.J, Coupling Freshly Isolated CD44<sup>+</sup> Infrapatellar Fat Pad-Derived Stromal Cells with a TGF-β3 Eluting Cartilage ECM-Derived Scaffold as a Single-Stage Strategy for Promoting Chondrogenesis, Advanced Healthcare Materials, 4, (7), 2015, p1043 - 1053
Luo L., Thorpe S.D., Buckley C.T. and Kelly D.J. , The effects of dynamic compression on the development of cartilage grafts engineered using bone marrow and infrapatellar fat pad derived stem cells, Biomedical Materials, 21, (10(5)), 2015, p055011
Mesallati T, Sheehy E.J, Vinardell T, Buckley C.T, Kelly D.J, Tissue engineering scaled-up, anatomically shaped osteochondral constructs for joint resurfacing, European Cells and Materials, 30, 2015, p163 - 186
Sridharan R, Reilly RB, Buckley CT, Decellularized grafts with axially aligned channels for peripheral nerve regeneration., Journal of the mechanical behavior of biomedical materials, 41, 2015, p124 - 135
Sheehy E.J., Mesallati T., Vinardell T., Kelly L., Buckley C.T. and Kelly D.J., Tissue engineering whole bones through endochondral ossification: Regenerating the distal phalanx, BioResearch, 4, (1), 2015, p229 - 241
Aisa, J., Calvo, I., Buckley, C.T., Kirby, B.M., Mechanical Comparison of Loop and Crimp Configurations for Extracapsular Stabilization of the Cranial Cruciate Ligament-Deficient Stifle, Veterinary Surgery, 44, (1), 2015, p50-58
Naqvi S.M. and Buckley C.T. , Bone Marrow-Derived Stem Cells in Response to Intervertebral Disc-Like Matrix Acidity - Implications for Cell-Based Regenerative Therapy, 4th Tissue Engineering and Regenerative Medicine International Society (TERMIS) World Congress, Boston, USA., 2015
Naqvi, S.M., Buckley, C.T., Differential response of encapsulated nucleus pulposus and bone marrow stem cells in isolation and coculture in alginate and chitosan hydrogels, Tissue Engineering - Part A, 21, (1-2), 2015, p288-299
Daniel John Kelly, Grainne Cunniffe, Henrique Almeida, Rajalakshmanan Eswaramoorthy, Conor Buckley, Peyno Pedro Diaz, 'A method for making a porous scaffold suitable for use in repair of osseous, chondral, or osteochondral defects in a mammal', 0, 2015
Naqvi, S.M. and Buckley, C.T. , Extracellular matrix production by nucleus pulposus and bone marrow stem cells in response to altered oxygen and glucose microenvironments, Journal of Anatomy, 227 , 2015, p757 - 766
Gilroy, D.A., O'Brien F.J., Buckley C.T. and Kearney C.J. , Magnetically active biomaterials for stem cell activation, UK Society for Biomaterials, University of Ulster, Northern Ireland, 2015
3D Printing and Additive Manufacturing for Biomedical Engineering in, editor(s)K. Kelly and G. O'Donnell , Perspectives on Manufacturing- Essays in honour of John Monaghan., 2015, pp85 - 96, [Buckley C.T. ]
Vedicherla S.V. and Buckley C.T, Tissue forming capacity of nasal and articular derived chondrocytes for intervertebral disc regeneration- Effect of oxygen and TGFβ stimulation, 4th Tissue Engineering and Regenerative Medicine International Society (TERMIS) World Congress, Boston, USA., 2015
Almeida HV, Liu Y, Cunniffe GM, Mulhall KJ, Matsiko A, Buckley CT, O'Brien FJ, Kelly DJ, Controlled release of transforming growth factor-ß3 from cartilage-extra-cellular-matrix-derived scaffolds to promote chondrogenesis of human-joint-tissue-derived stem cells., Acta biomaterialia, 10, (10), 2014, p4400-9
Liu Y, Buckley CT, Almeida HV, Mulhall KJ, Kelly DJ, Infrapatellar Fat Pad-Derived Stem Cells Maintain Their Chondrogenic Capacity in Disease and Can be Used to Engineer Cartilaginous Grafts of Clinically Relevant Dimensions., Tissue engineering. Part A, 20, (21-22), 2014, p3050-62
Naqvi, S.M., Guillaume, O., and Buckley C.T, Oxygen is a More Potent Regulator than Glucose Concentration for Matrix Production of Nucleus Pulposus and Bone Marrow-derived Stem Cells, 60th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, USA, 2014
Mesallati T, Buckley CT, Kelly DJ, A comparison of self-assembly and hydrogel encapsulation as a means to engineer functional cartilaginous grafts using culture expanded chondrocytes., Tissue engineering. Part C, Methods, 20, (1), 2014, p52-63
Almeida H.V., Liu, Y. Cunniffe G.M., Mulhall K.J., Matsiko A., Buckley C.T., O'Brien F.J. and Kelly D.J. , Controlled release of TGF-β3 from cartilage extra cellular matrix derived scaffolds to promote chondrogenesis of human joint tissue derived stem cells, Acta Biomaterialia , 10, (10), 2014, p4400 - 4409
Kearney C.M., Buckley C.T., Jenner F and Brama P.A.J. , Elasticity and Breaking Strength of Synthetic Suture Materials Incubated in Various Equine Physiological and Pathological Solutions, Equine Veterinary Journal , 46, (4), 2014, p494 - 498
Naqvi S.M. and Buckley C.T. , Response of Bone Marrow-Derived Stem Cells Cultured in Intervertebral Disc-Like Microenvironmental Conditions, Tissue Engineering and Regenerative Medicine International Society (TERMIS), Genova, Italy, 2014
Kelly, L.J., Guillaume O., Naqvi S.M., Robinson A.J. and Buckley C.T., Cell Microencapsulation Using Electrohydrodynamic Spraying for Minimally Invasive Tissue Repair, Tissue Engineering and Regenerative Medicine International Society (TERMIS), Genova, Italy, 2014
Kelly, L.J., Naqvi S.M. and Buckley C.T. , Microencapsulation of bone marrow derived stem cells using electrohydrodynamic spraying for minimally invasive tissue repair of the IVD, eCM XV: Cartilage & Disc: Repair and Regeneration, Davos, Switzerland, 2014
Carroll, S.F., Buckley, C.T., Kelly, D.J., Cyclic Hydrostatic Pressure Promotes A Stable Cartilage Phenotype And Enhances The Functional Development Of Cartilaginous Grafts Engineered Using Multipotent Stromal Cells Isolated From Bone Marrow And Infrapatellar Fat Pad. , Journal of Biomechanics, 47, (9), 2014, p2115 - 2121
Mesallati,Tariq T., Buckley,Conor Timothy C.T., Kelly,Daniel John D.J., Engineering articular cartilage-like grafts by self-assembly of infrapatellar fat pad-derived stem cells, Biotechnology and Bioengineering, 111, (8), 2014, p1686-1698
Baboolal T.G., Boxall S.A., Churchman S.M., Buckley C.T., Jones E. and McGonagle D. , Intrinsic multipotential mesenchymal stromal cell activity in gelatinous Heberden's nodes in osteoarthritis at clinical presentation., Arthritis Research & Therapy , 16, (3), 2014, pR19
Guillaume, O., Gansau, J., Lennon, K., Quinn, S.J., Kaye-Coyle, R. and Buckley C.T. , Fabrication of Porous Scaffolds Derived from Decellularized Annulus Fibrosus Extracellular Matrix, 60th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, USA, 2014
Guillaume, O., Daly, A., Lennon K., Gansau J., Buckley S.F. and Buckley, C.T., Shape-memory porous alginate scaffolds for regeneration of the annulus fibrosus - Effect of TGF-β3 supplementation and oxygen culture conditions, Acta Biomaterialia, 10, 2014, p1985 - 1991
Sridharan, R., Reilly, R.B. and Buckley C.T. , Decellularized Grafts with Longitudinal Oriented Channels for Peripheral Nerve Regeneration, 60th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, USA, 2014
Sheehy E.J., Mesallati T., Buckley C.T. and Kelly D.J. , Anatomic Hypertrophic Cartilaginous Grafts For Whole Bone Tissue Engineering, 60th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, USA, 2014
Mesallati T., Sheehy E.J., Vinardell T., Buckley C.T. and Kelly D.J., Tissue Engineering Scaled-Up, Anatomically Accurate, Osteochondral Constructs for Joint Resurfacing, 60th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, USA, 2014
Sheehy EJ, Vinardell T, Toner ME, Buckley CT, Kelly DJ, Altering the architecture of tissue engineered hypertrophic cartilaginous grafts facilitates vascularisation and accelerates mineralisation., PloS one, 9, (3), 2014, pe90716
Sheehy EJ, Vinardell T, Buckley CT, Kelly DJ, Engineering osteochondral constructs through spatial regulation of endochondral ossification., Acta biomaterialia, 9, (3), 2013, p5484-5492
Mesallati, T., Buckley, C.T., Nagel, T., Kelly, D.J., Scaffold architecture determines chondrocyte response to externally applied dynamic compression. , Biomechanics and Modeling in Mechanobiology, 12, (5), 2013, p889-899
Guillaume, O., Daly, A., Naqvi, S.M. and Buckley, C.T. , Development of Shape-Memory Porous Scaffolds for Minimal Invasive Repair of the Annulus Fibrosus, Tissue Engineering and Regenerative Medicine International Society (TERMIS), Istanbul, Turkey, 2013
Naqvi, S.M., Tryfon, M.K. and Buckley, C.T. , Determining Optimal Cell Ratios of Nucleus Pulposus and Mesenchymal Stem Cells Co-Cultured in a Nutrient Deprived Microenvironment, Tissue Engineering and Regenerative Medicine International Society (TERMIS), Istanbul, Turkey, 2013
Sheehy E.J., Vinardell T., Buckley C.T. and Kelly D.J. , Towards Engineering Whole Bones via Endochondral Ossification, ASME 2013 Summer Bioengineering Conference, Oregon, USA, 2013
Maguire S.C., Naqvi S.M., Tryfon M.E. and Buckley C.T. , Co-Culture of Nucleus Pulposus and Mesenchymal Stem Cells in a Nutrient Limited In Vitro System- Implications for Intervertebral Disc Regeneration Strategies, Annual Meeting of the Orthopaedic Research Society, San Antonio, Texas, USA, 2013
Carroll, S.F., Buckley, C.T. and Kelly, D.J. , The influence of hydrostatic pressure on the functional development & phenotypic stability of cartilaginous grafts engineered using mesenchymal stem cells isolated from bone marrow & infrapatellar fat pad, Annual Meeting of the Orthopaedic Research Society, San Antonio, Texas, USA, 2013
O'Heireamhoin S, Buckley CT, Jones E, McGonagle D, Mulhall KJ, Kelly DJ, Recapitulating Aspects of the Oxygen and Substrate Environment of the Damaged Joint Milieu for Stem Cell-Based Cartilage Tissue Engineering., Tissue engineering. Part C, Methods, 19, (2), 2013, p117-127
Liu, Y., Buckley, C.T., Mulhall, K.J., Kelly, D.J., Combining BMP-6, TGF-β3 and hydrostatic pressure stimulation enhances the functional development of cartilage tissues engineered using human infrapatellar fat pad derived stem cells, Biomaterials Science, 1, (7), 2013, p745-752
Almeida H.V., Liu, Y. Cunniffe G.M., Buckley C.T., O'Brien F.J. and Kelly D.J. , The Composition and Architecture of Cartilage ECM Derived Scaffolds Regulates Stem Cell Infiltration and their Chondrogenic Differentiation, Tissue Engineering and Regenerative Medicine International Society (TERMIS), Istanbul, Turkey, 2013
Luo L., Thorpe S.D., Buckley C.T. and Kelly D.J., Engineering zonal cartilaginous tissue using infrapatellar fat pad derived stem cells by modulating the spatial environment within the developing construct, Annual Meeting of the Orthopaedic Research Society, San Antonio, Texas, USA , 2013
Buckley, C.T., Ahearne, M., Downey R., Mulhall K.J. and Kelly D.J., Engineering Cartilage Grafts using Fibrin Hydrogels Seeded with Diseased Human Infrapatellar Fat Pad Derived Stem Cells, Annual Meeting of the Orthopaedic Research Society, San Francisco, California, USA, 2012
Almeida H.V., Buckley C.T., Cunniffe G.M., Ahearne M., O'Brien F.J. and Kelly D.J., Combining freshly isolated CD271+ positive infrapatellar fat pad stem cells and a TGF-β3 releasing scaffold to develop a single stage therapy for cartilage repair, Tissue Engineering and Regenerative Medicine International Society (TERMIS), Hofburg Congress Centre, Vienna, Austria, 2012
Sheehy E.J., Vinardell T., Buckley C.T. and Kelly D.J. , Engineering Osteochondral Constructs through Spatial Regulation of Endochondral Ossification, Annual Meeting of the Orthopaedic Research Society, San Francisco, California, USA, 2012
Vinardell T., Rolfe R.A., Buckley C.T., Meyer E.G., Murphy P. and Kelly D.J. , Hydrostatic Pressure Plays a Role in Both the Induction and Maintenance of a Chondrogenic Phenotype in Joint Tissue Derived Stem Cells, Annual Meeting of the Orthopaedic Research Society, San Francisco, California, USA, 2012
C.T. Buckley, D.J. Kelly, Expansion in the presence of FGF-2 enhances the functional development of cartilaginous tissues engineered using infrapatellar fat pad derived MSCs, Journal of the Mechanical Behavior of Biomedical Materials, 11, 2012, p102-111
Liu Y, Buckley CT, Downey R, Mulhall KJ, Kelly DJ, The role of environmental factors in regulating the development of cartilaginous grafts engineered using osteoarthritic human infrapatellar fat pad-derived stem cells., Tissue engineering. Part A, 18, (15-16), 2012, p1531-1541
Sheehy E.J., Vinardell T., Buckley C.T. and Kelly D.J. , Modulating Endochondral Ossification to Engineer Osteochondral Constructs, 18th Congress of the European Society of Biomechanics, Lisbon, Portugal, 2012
Carroll, S.F., Buckley, C.T. and Kelly, D.J. , Oxygen consumption during chondrogenesis in hydrogels seeded with chondrocytes and stem cells isolated from different tissues, Tissue Engineering and Regenerative Medicine International Society (TERMIS), Hofburg Congress Centre, Vienna, Austria, 2012
Thorpe S.D., Buckley C.T., Steward A.J. and Kelly D.J. , Extrinsic biophysical stimulus can override the influence of local substrate in determining stem cell fate, Tissue Engineering and Regenerative Medicine International Society (TERMIS), Hofburg Congress Centre, Vienna, Austria, 2012
Liu Y, Buckley C.T., Downey R., Mulhall K and Kelly D.J. , The role of environmental factors in regulating the development of cartilaginous tissues engineered using osteoarthrithic human infrapatellar fat pad derived stem cells., Annual Meeting of the Orthopaedic Research Society, San Francisco, California, USA, 2012
Vinardell T, Rolfe RA, Buckley CT, Meyer EG, Ahearne M, Murphy P, Kelly DJ, Hydrostatic pressure acts to stabilise a chondrogenic phenotype in porcine joint tissue derived stem cells., European Cells & Materials, 23, 2012, p121-134
A.J. Steward, S.D. Thorpe, C.T. Buckley, D.R. Wagner, D.J. Kelly, Cell-matrix interactions regulate mesenchymal stem cell response to hydrostatic pressure, Acta Biomaterialia, 8, (6), 2012, p2153-2159
Mesallati T., Buckley C.T. and Kelly D.J. , Engineering functional cartilaginous grafts using chondrocyte agarose constructs supported by a superficial layer of stem cells, Tissue Engineering and Regenerative Medicine International Society (TERMIS), Hofburg Congress Centre, Vienna, Austria, 2012
Cox G., Boxhall S., Giannoudis P.V., Buckley C.T., Roshdy T., Churchman S.M., McGonagle D. and Jones E.: , High abundance of CD271+ multipotential stromal cells (MSCs) in intramedullary cavities of long-bones, Bone, 50, (2), 2012, p510-517
Vinardell T, Sheehy EJ, Buckley CT, Kelly DJ, A comparison of the functionality and in vivo phenotypic stability of cartilaginous tissues engineered from different stem cell sources., Tissue engineering. Part A, 18, (11-12), 2012, p1161-1170
Buckley CT, Meyer EG, Kelly DJ, The influence of construct scale on the composition and functional properties of cartilaginous tissues engineered using bone marrow-derived mesenchymal stem cells., Tissue engineering. Part A, 18, (3-4), 2012, p382-396
Vinardell T., Sheehy E.J., Buckley C.T. and Kelly D.J. , A comparison of the functionality and in vivo phenotypic stability of cartilaginous tissues engineered from different stem cell sources, Annual Meeting of the Orthopaedic Research Society, San Francisco, California, USA, 2012
Sheehy EJ, Buckley CT, Kelly DJ, Oxygen tension regulates the osteogenic, chondrogenic and endochondral phenotype of bone marrow derived mesenchymal stem cells., Biochemical and biophysical research communications, 417, (1), 2012, p305-310
O'Eireamhoin S. *, Buckley C.T. *, Schepens A, Jones E, McGonagle D, Mulhall K.J. and Kelly D.J., The effects of growth factor supplementation and oxygen tension during expansion on subsequent chondrogenesis of human infrapatellar fat pad derived mesenchymal stem cells, Journal of Bone & Joint Surgery, British Volume 94 (SUPP XXXIX), 2012
Haugh, M.G., Thorpe, S.D., Vinardell, T., Duffy, G.P. and Kelly, D.J. , The application of plastic compression to modulate fibrin hydrogel mechanical properties., Journal of the Mechanical Behavior of Biomedical Materials, 16, 2012, p66-72
Liu Y., Buckley C.T., Downey R., Mulhall K. and Kelly D.J., Chemical and physical cues to regulate the development of cartilaginous tissues engineered using osteoarthritic human infrapatellar fat pad derived stem cells, Tissue Engineering and Regenerative Medicine International Society (TERMIS), Hofburg Congress Centre, Vienna, Austria, 2012
Thorpe SD, Buckley CT, Steward AJ, Kelly DJ, European Society of Biomechanics S.M. Perren Award 2012: The external mechanical environment can override the influence of local substrate in determining stem cell fate., Journal of biomechanics, 45, (15), 2012, p2483-2492
Sheehy E.J., Vinardell T., Buckley C.T. and Kelly D.J., Channelled architectures accelerate in vivo mineralisation of engineered hypertrophic cartilaginous constructs, Tissue Engineering and Regenerative Medicine International Society (TERMIS), Hofburg Congress Centre, Vienna, Austria, 2012
Liu Y., Mesallati T., Buckley C.T., Downey R., Mulhall K. and Kelly D.J. , Scaffold-free cartilaginous tissue engineered using osteoarthritic human infrapatellar fat pad derived stem cells, Tissue Engineering and Regenerative Medicine International Society (TERMIS), Hofburg Congress Centre, Vienna, Austria, 2012
T. Vinardell, C.T. Buckley, S. D. Thorpe, and D. J. Kelly, Composition-function relations of cartilaginous tissues engineered from chondrocytes and mesenchymal stem cells isolated from bone marrow and infrapatellar fat pad, Journal of Tissue Engineering and Regenerative Medicine, 5, (9), 2011, p673-683
Cox G., McGonagle D., Boxhall S.A., Buckley C.T., Jones E. and Giannoudis P.V., The use of the reamer-irrigator-aspirator to harvest mesenchymal stem cells., Journal of Bone and Joint Surgery, 93-B, (4), 2011, p517-524
Vinardell T., Buckley C.T., Meyer E.G. and Kelly D.J. , Hydrostatic pressure and TGF-beta3 interact to regulate chondrogenesis of joint derived mesenchymal stem cells., Tissue Engineering and Regenerative Medicine International Society (TERMIS), Granada, Spain, 7th-10th June, 2011
Sheehy, E.J., Vinardell, T., Meyer, E.G., Buckley, C.T. and Kelly, D.J. , The oxygen environment regulates mesenchymal stem cell expansion and chondrogenic differentiation in an in vitro cartilage defect model., Annual Meeting of the Orthopaedic Research Society, Long Beach, California, USA, January 13th-16th, 2011
Sheehy E.J., Buckley C.T. and Kelly D.J., Chondrocytes and bone marrow-derived mesenchymal stem cells undergoing chondrogenesis in agarose hydrogels of solid and channelled architectures respond differentially to dynamic culture conditions., Tissue Engineering & Regenerative Medicine., 5, (9), 2011, p747-758
Buckley, C.T., Meyer, E.G. and Kelly, D.J. , Expansion in the Presence of FGF-2 Modulates the Temporal Development of Cartilaginous Tissues Engineered Using Infrapatellar Fat Pad Derived MSCs, Annual Meeting of the Orthopaedic Research Society, Long Beach, California, USA, January 13th-16th, 2011
Meyer, E.G., Buckley, C.T., and Kelly, D.J. , The Effect of Cyclic Hydrostatic Pressure on the Functional Development of Cartilaginous Tissues Engineered Using Bone Marrow Derived Mesenchymal Stem Cells., ASME 2011 Summer Bioengineering Conference, Pennsylvania, US, June 22nd-25th, ASME, 2011
Mesallati, T., Buckley, C.T., Nagel, T., and Kelly, D.J. , Scaffold Architecture Determines Chondrocyte Response to Externally Applied Dynamic Compression, Annual Meeting of the Orthopaedic Research Society, Long Beach, California, USA, 2011
Ahearne M, Buckley CT, Kelly DJ, A growth factor delivery system for chondrogenic induction of infrapatellar fat pad-derived stem cells in fibrin hydrogels., Biotechnology and Applied Biochemistry, 58, (5), 2011, p345-352
Mesallati T., Buckley C.T., Nagel T. and Kelly D.J., Introducing microchannels into chondrocyte-seeded agarose hydrogels influences matrix accumulation in response to dynamic compression and TGF-â3 stimulation., IFMBE Proceedings , 8th International Conference on Cell & Stem Cell Engineering (ICCE) , 30, 2011, pp26-30
Thorpe S.D., Buckley C.T., Steward, A.J and Kelly D.J. , Cell-Matrix Interactions Modulate Mesenchymal Stem Cell Response To Dynamic Compression, ASME 2011 Summer Bioengineering Conference, Pennsylvania, USA, June 22nd-25th, ASME, 2011
E.G. Meyer, C.T. Buckley, A.J. Steward, D.J. Kelly, The effect of cyclic hydrostatic pressure on the functional development of cartilaginous tissues engineered using bone marrow derived mesenchymal stem cells, Journal of the Mechanical Behavior of Biomedical Materials, 4, (7), 2011, p1257-1265
Steward A.J., Thorpe S.D., Vinardell T., Buckley C.T., Wagner D.R. and Kelly D.J., Cell-Matrix interactions regulate mesenchymal stem cell response to extrinsic mechanical signals, European Society for Biomaterials, Dublin, Ireland, 2011
Thorpe S.D., Buckley C.T., and Kelly D.J., Can dynamic compression in the absence of growth factors induce chondrogenic differentiation of bone marrow derived MSCs encapsulated in agarose hydrogels?, IFMBE Proceedings, IFMBE, 30, 2011, pp43-46
Ahearne M.A., Buckley C.T. and Kelly D.J., Engineering a MSC seeded fibrin hydrogel containing TGF-beta 1 loaded gelatin microspheres for cartilage repair, Tissue Engineering and Regenerative Medicine International Society (TERMIS), Granada, Spain, 2011
Mesallati, T., Buckley, C.T. and Kelly, D.J. , Is self assembly using progenitor cells a better approach to engineering functional cartilage tissue than hydrogel encapsulation? , Tissue Engineering and Regenerative Medicine International Society (TERMIS), Granada, Spain, 2011
Meyer, E.G., Buckley, C.T., and Kelly, D.J. , The Functional Properties of Cartilaginous Tissues Engineered Using Bone-Marrow Derived MSCs Improve As They Are Scaled Towards Geometries of Clinical Relevance. , Annual Meeting of the Orthopaedic Research Society, Long Beach, California, USA., January 13th-16th, 2011
Meyer E.G., Buckley C.T., Thorpe S.D. and Kelly D.J., Low oxygen tension: A more potent chondrogenic stimulus than dynamic compression for bone marrow derived MSCs in agarose hydrogel culture., 56th Annual Meeting of the Orthopaedic Research Society, Morial Convention Center Hall H, New Orleans, Louisiana, USA, March 6 - 9, 2010
Buckley, C T; Vinardell, T; Thorpe, S D; Jones, E; McGonagle, D; Kelly, D J. , Functional Properties of Cartilaginous Tissues Engineered From Infrapatellar Fat Pad Derived Mesenchymal Stem Cells. , Journal of Biomechanics , 43, (5), 2010, p920-926
Meyer EG, Buckley CT, Thorpe SD, Kelly DJ., Low oxygen tension is a more potent promoter of chondrogenic, Journal of Biomechanics, 43, (13), 2010, p2516-2523
Cox G., Giannoudis P.V., Boxall S., Buckley C.T., Jones E., and McGonagle D, Extraction of high numbers of mesenchymal stem cells (MSCs) from intramedullary cavities of long bones, Injury Extra, 41, (12), 2010, pp155 - 156
Cox G, McGonagle D, Boxhall S., Buckley C.T., Jones E. and Giannoudis P.V. , The Reamer- Irrigator-Aspirator (RIA): A harvester of mesenchymal stem cells (MSCs)., 4th UK Mesenchymal Stem Cell Meeting, University of Leeds, UK, 14th April, 2010
Buckley C.T., Vinardell T. and Kelly D.J., Infrapatellar Fat Pad Derived MSCs as a Potential Alternative to Chondrocytes for Cartilage Repair- Chondrogenesis in a Physiologically Relevant Low Oxygen Tension Environment., 4th UK Mesenchymal Stem Cell Meeting, University of Leeds, UK, 14th April, 2010
Thorpe, S. D., Buckley, C. T., Vinardel, T., O'Brien, F. J., Campbell, V. A., Kelly, D. J. , The response of bone marrow derived mesenchymal stem cells to dynamic compression following TGF-â3 induced chondrogenic differentiation, Annals of Biomedical Engineering , 38, (9), 2010, p2896-2909
Buckley C.T., O'Kelly K.U, Fabrication and characterization of a porous multidomain hydroxyapatite scaffold for bone tissue engineering investigations, Journal of Biomedical Materials Research, 93B, (2), 2010, p459 - 467
Sheehy, E.J., Buckley C.T., and Kelly D.J. , Cartilaginous tissue engineering using chondrocytes and bone marrow derived MSCs by a combination of rotational culture and modified scaffold architecture, Tissue Engineering and Regenerative Medicine International Society - EU Meeting, Galway, Ireland,, 2010
Thorpe S.D., Buckley C.T., Vinardell T., O'Brien F.J., Campbell V.A. and Kelly D.J., Dynamic Compression Post TGF-â3 Induced Differentiation Augments Chondrogenesis of Mesenchymal Stem Cells., 56th Annual Meeting of the Orthopaedic Research Society, Morial Convention Center Hall H, New Orleans, Louisiana, USA, March 6 - 9, 2010
Meyer E.G., Buckley C.T., Haugh M.G. and Kelly D.J., The influence of expansion on the resulting functional properties of cartilaginous tissues generated from MSCs., 4th UK Mesenchymal Stem Cell Meeting, University of Leeds, UK, 14th April , 2010
Buckley CT, Vinardell T, Kelly DJ., Oxygen tension differentially regulates the functional properties of cartilaginous tissues engineered from infrapatellar fat pad derived MSCs and articular chondrocytes., Osteoarthritis Cartilage., 18, (10), 2010, p1345-1354
Mesallati, T.S., Buckley, C.T., Kelly, D.J., The effect of introducing microchannels into dynamically compressed chondrocyte-seeded hydrogels, 8th International Conference on Cell & Stem Cell Engineering (ICCE), , Dublin, 11-12th June 2010. , 2010
Meyer EG, Buckley CT, Thorpe SD, Kelly DJ., Low oxygen tension: A more potent chondrogenic stimulus than dynamic compression for bone marrow derived MSCs in agarose hydrogel culture., . 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, LA, March 6 - 9, 2010
Vinardell T, Buckley C, Thorpe S, Kelly D, Functional properties of cartilaginous tissues generated from mesenchymal stem cells isolated from different tissue sources., 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, USA , 2010
McAlinden A.B., Buckley C.T., and Kirby B.M. , Biomechanical evaluation of different numbers, sizes and placement configurations of ligaclips required to secure cellophane bands, Veterinary Surgery, 39, (1), 2010, p59-64
Meyer EG, Haugh MG, Buckley CT, Kelly DJ., Monolayer expansion and cell seeding density influence the functional properties of cartilaginous tissues engineered using MSCs., Orthopedic Research Society Meeting, New Orleans, LA, 2010
Thorpe S.D., Buckley C.T., Vinardell T., O'Brien F.J., Campbell V.A. and Kelly D.J., Dynamic compression of mesenchymal stem cells following cytokine induced differentiation augments glycosaminoglycan synthesis., 4th UK Mesenchymal Stem Cell Meeting, University of Leeds, UK, 14th April, 2010
Meyer E.G., Buckley C.T., Kelly D.J, The Effect of Cyclic Hydrostatic Pressure and Low Oxygen Tension on the Chondrogenic Differentiation of Bone Marrow Derived Mesenchymal Stem Cells., 8th International Conference on Cell & Stem Cell Engineering (ICCE), , Dublin, 11-12th June, 2010
Meyer E.G., Haugh M.G., Buckley C.T. and Kelly D.J. , Monolayer expansion and cell seeding density influence the functional properties of cartilaginous tissues engineered using MSCs., 56th Annual Meeting of the Orthopaedic Research Society, Morial Convention Center Hall H, New Orleans, Louisiana, March 6 - 9, 2010
Buckley C.T., O'Kelly K.U., Maintaining cell depth viability: on the efficacy of a trimodal scaffold pore architecture and dynamic rotational culturing., Journal of Materials Science: Materials in Medicine, 21, (5), 2010, p1731-1738
Buckley, C.T., Thorpe, S.D., Kelly, D.J. , Engineering of large cartilaginous constructs through the use of microchanneled hydrogels and rotational culture, Tissue Engineering A, 15, (11), 2009, p3213-3220
Vinardell, T; Thorpe, S D; Buckley, C T; Kelly, D J, Chondrogenesis and integration of mesenchymal stem cells within an in vitro cartilage defect repair model, Annals of Biomedical Engineering, 37, (12), 2009, p2556-2565
Thorpe, S., Vinardell, T., Buckley, C., Kelly, D.J., Dynamic Compressive Loading can Inhibit Chondrogenesis of Bone Marrow Derived Mesenchymal Progenitor Cells, Trans. 55th Annual Meeting of the Orthopaedic Research Society, Las Vegas, 2009
Vinardell T, Buckley C, Thorpe S, Kelly D , Functional properties of cartilaginous tissues generated from mesenchymal stem cells isolated from different tissue sources., European Cells and Materials , X European Cells and Materials Conference, Stem Cells for musculoskeletal regeneration, Davos, Switzerland, June 29- July 1, 18, (1), 2009, pp63
Thorpe S.D., Buckley C.T., Vinardell T., O'Brien F.J., Campbell V.A. and Kelly D.J., Dynamic compression can inhibit chondrogenesis of mesenchymal stem cells, 3rd UK Mesenchymal Stem Cell Meeting, University of Sheffield, United Kingdom, 2009
Thorpe, S., Buckley, C., Kelly, D.J., Chondrogenesis of Mesenchymal Stem Cells is Differentially Regulated by Temporal Application of Dynamic Compression, ECM X: Stem Cells for Musculoskeletal Regeneration, Davos, Switzerland, 2009
Vinardell T, Buckley C.T. and Kelly D.J. , Mechanical Behaviour of Cartilaginous Tissues Engineered from Mesenchymal Stem Cells Isolated from Different Tissue Sources, Third International Conference on Mechanics of Biomaterials and Tissues, Clearwater Beach, Florida, USA, 13 - 17 December , 2009
Vinardell, T., Thorpe, S., Buckley, C., Kelly, D.J., Chondrocytes demonstrate superior integration than mesenchymal stem cells in a cartilage explant defect model., Trans. 55th Annual Meeting of the Orthopaedic Research Society, Las Vegas, 2009
Buckley, C.T., Thorpe, S.D., O'Brien, F.J., Robinson, A.J., Kelly, D.J. , The effect of concentration, cooling rate and cell seeding density on the initial mechanical properties of agarose hydrogels., Mechanical Behaviour of Biomedical Materials , 2, (5), 2009, p512 - 521
Vinardell T, Thorpe S, Buckley C, Kelly D. , Chondrogenesis of bone marrow mesenchymal stem cells and chondrocytes- an in vitro cartilage defect repair model., 3rd UK Mesenchymal Stem Cell Meeting, Sheffield, UK, 2009
Haut Donadue, T., Thorpe, S., Buckley, C., Kelly, D.J., The Response of Inner vs. Outer Zone Meniscal Cells to Unconfined Compression. , Trans. 55th Annual Meeting of the Orthopaedic Research Society, Las Vegas, 2009
Buckley, C., Thorpe, S., Kelly, D.J. , The Effect of Dynamic Rotational Culturing on Chondrocytes in a Microchannel Agarose Hydrogel Environment., Trans. 55th Annual Meeting of the Orthopaedic Research Society, Las Vegas, 2009
Vinardell T, Thorpe S, Buckley C, Kelly D., Chondrogenesis of bone marrow mesenchymal stem cells and chondrocytes- an in vitro cartilage defect repair model., 55th Annual Meeting of the Orthopaedic Research Society, Las Vegas, USA, 2009
Vinardell T, Buckley C, Thorpe S, Kelly D, Functional properties of cartilaginous tissues generated from mesenchymal stem cells isolated from different tissue sources., 3rd International Conference on Mechanics of Biomaterials and Tissues, Clearwater Beach, USA, 2009
Kelly, K., Buckley, C., O'Donnell, G.E., Ervine, P., Surface Finish in Micro-drilling of PTFE, 25th International Manufacturing Conference, Dublin Institute of Technology, 3 - 5 Sept, edited by Robert Simpson , 2008, pp65 - 72
Thorpe, S. D., Buckley, C. T., Vinardell, T., O'Brien, F. J., Campbell, V. A., Kelly, D. J. , Dynamic compression can inhibit chondrogenesis of mesenchymal stem cells. , Biochemical and Biophysical Research Communications, 337, (2), 2008, p458-462
Buckley, C.T., O'Brien F.J., Robinson, A., Kelly, D.J., 'Cell/Hydrogel mixing and delivery - Thermally regulated injection device', 0, EP 08394020.5, 2008
Thorpe S.D., Buckley C.T., O'Brien F.J., Robinson A.J. and Kelly D.J., Fabrication Methodologies Regulate the Initial Mechanical Properties Of Cell Seeded Hydrogels, 16th Conference of the European Society of Biomechanics, Lucerne, Switzerland, 2008
Buckley, C.T. and O'Kelly, K.U. , Development of a Hydroxyapatite Bone Tissue Engineering Scaffold with a Trimodal Pore Structure, Key Engineering Materials , 361-363, 2008, p931 - 934
Regular Scaffold Fabrication Techniques for Investigations in Tissue Engineering in, editor(s)P.J. Prendergast & P.E McHugh , Topics in Biomechanical Engineering, 2004, pp147 - 166, [C.T. Buckley and K.U. O'Kelly]
Non-Peer-Reviewed Publications
Gansau J., McDonnell E. and Buckley, C.T. , Enhancing the local acidic microenvironment found in degenerated intervertebral discs using pH neutralizing microcapsules, Proceedings of the 26th Annual Conference of the Section of Bioengineering of the Royal Academy of Medicine in Ireland, Mount Wolseley Hotel, Spa & Golf Resort, Co Carlow, Ireland , 2020
Ryan T. and Buckley, C.T. , Ambient temperature transportation of chondrocytes for cell therapies, 24th Annual Conference of the Section of Bioengineering of the Royal Academy of Medicine in Ireland, Johnstown Estate Hotel & Spa, Enfield, Co. Meath , 2018
Tornifoglio B., Kerskens C.M. and Buckley C.T., Non-invasive characterisation of the intervertebral disc microenvionment through finite element modelling and magnetic resonance imaging, 24th Annual Conference of the Section of Bioengineering of the Royal Academy of Medicine in Ireland, Johnstown Estate Hotel & Spa, Enfield, Co. Meath , 2018
Mandal, P. and Buckley, C.T., Comparison of bone marrow stem cells and nasal chondrocytes for nucleus pulposus regeneration, Proceedings of the 23rd Annual Conference of the Section of Bioengineering of the Royal Academy of Medicine in Ireland, 2017
Irwin, P.P. and Buckley, C.T. , Glucose mapping of bovine intervertebral disc organ culture models. An experimental and numerical analysis. , Proceedings of the 23rd Annual Conference of the Section of Bioengineering of the Royal Academy of Medicine in Ireland, 2017
Buckley C.T. , SCIENCE & SPORTS INJURIES- How tissue engineering will reduce the need for complex surgery?, A World of Science in Sport, Public Lecture, Global Room, Trinity College Dublin, Jul , 2014
Buckley C.T. , Regenerating the Intervertebral Disc- Biomaterial Strategies, Stem Cells and Microenvironmental Effects, Icahn School of Medicine at Mount Sinai, New York, USA, Nov , 2014
Buckley C.T. , Regenerative Medicine & Tissue Engineering, Trinity Centre for Bioengineering Winter Symposium, Trinity College Dublin, Dec , 2010
Buckley C.T. , Articular Cartilage Repair- Biophysical and Biochemical Environments for Mesenchymal Stem Cell Based Therapies, Seminar Series, University of Sheffield, UK, May , 2010
Sheehy E.J., Buckley C.T. and Kelly D.J., Rotational culture differentially regulates chondrogenesis of bone marrow derived MSCs and chondrocytes., Proceedings of the 16th Annual Conference of the Section of Bioengineering of the Royal Academy of Medicine in Ireland. , 2010
Vinardell T, Buckley C, Thorpe S, Kelly D, Functional properties of cartilaginous tissues generated from mesenchymal stem cells isolated from different tissue sources., 16th Annual Conference of the Section of Bioengineering, Dublin, Ireland, 2010
Buckley C.T. , Mesenchymal Stem Cell Based Therapies for Articular Cartilage Repair, Mesenchymal Stem Cell Group Seminar Series, University of Leeds, July , 2010
Buckley C.T. , Mechanobiology of mesenchymal stem cells, Stem Cell Workshop, Trinity College Dublin, Mar, 2009
Vinardell T, Thorpe S, Buckley C, Kelly D. , Chondrogenesis of bone marrow mesenchymal stem cells and chondrocytes- an in vitro cartilage defect repair model., 15th Annual Conference of the Section of Bioengineering, Limerick, Ireland, edited by McGloughlin and M. Walsh , 2009
Buckley C.T. , Regenerative Medicine & Tissue Engineering, Trinity Centre for Bioengineering Winter Symposium, Trinity College Dublin, Dec, 2009
Buckley C.T. & O'Kelly K.U., Fabrication of a hydroxyapatite scaffold with a tri-modal pore distribution for bone tissue engineering, Proceedings of the 12 Annual Conference of the Section of Bioengineering of the Royal Academy of Medicine in Ireland, Galway, edited by McHugh P.E., O'Mahoney, D. and Fitzpatrick D. , 2006, pp89
Buckley C.T., Prina-Mello A., O'Kelly K.U., Development of biodegradable elastomeric scaffolds for mechanobiology investigations, Proceedings of the 11th Annual Conference of the Section of Bio-engineering of the Royal Academy of Medicine in Ireland, Galway, edited by FitzPatrick D.P. and Senouci M. , 2005, pp13-
Buckley C.T. and O'Kelly K.U., A new technique for fabricating regular 3D scaffolds for bone tissue engineering applications, Proceedings of the tenth Annual Conference of the Section of Bioengineering of the Royal Academy of Medicine in Ireland, Limerick, edited by D.P. Fitzpatrick, B.A.O. McCormack and T.M. McGlouhlin , 2004, pp19-
Research Expertise
Projects
- Title
- Evaluation of Bioinks from Human-Origin Biomaterials for Enhanced Wound Regeneration (BIOHEAL)
- Summary
- Due to a growing active aging population and the increased incidence of global diseases wound repair remains a significant challenge in clinical settings, necessitating innovative approaches to develop advanced wound care products and enhance healing outcomes. This proposal seeks to investigate the potential of human origin biomaterials to biofabricate wound care products leveraging the intrinsic properties of platelet lysates such as cytokine factors to promote healing and the antimicrobial activity of keratin-derived biomaterials for infection control.
- Funding Agency
- AMBER and Science Foundation Ireland
- Title
- Development of Biomimetic Vessels for Surgical Training
- Summary
- Surgical training plays a crucial role in preparing surgeons for complex procedures using medical devices. Traditional surgical training methods often rely on cadavers and animal models with associated ethical challenges. The development of biomimetic vessels, which accurately mimic human structure and function, has the potential to enhance surgical training by providing a more realistic and ethical alternative.
- Funding Agency
- AMBER, Science Foundation Ireland and Johnson and Johnson
- Title
- The Oestrogen-Adipokine Axis and Its Influence on Vertebral Cartilaginous End-Plate Calcification and Porosity Formation in Pre-Menopausal Females
- Summary
- The global spread of obesity has been labelled a pandemic and has tripled in incidence since 1975. A major concern is the effect obesity has on low back pain (LBP), which already exceeds healthcare costs of 240 billion per year in Europe. If not addressed appropriately, with concerted efforts into appropriate treatments, the obesity pandemic and ever-growing incidence of LBP could cripple the healthcare system. The classical model or theory is that obesity influences LBP via altered distribution of forces in the spine, due to the increasing amount of abdominal fat with age. This theory is flawed for several reasons. Firstly, a notable proportion of patients presenting with LBP are young patients, reported as high as 30% of presentations. Secondly, young females store more of their fat in their thigh region. Thus, previous theories do not apply to young females and the significant proportion of LBP patients they represent. It is the premise of this proposal that the true causation of LBP in young females stems from the presence of fat tissue cells, known as adipokines, influenced by levels of oestrogen. Younger females have been shown to have higher levels of these cells than both males and elderly females. It is believed this is due to the differences in levels of oestrogen. Thus, the objective of this proposal is to ascertain the link between oestrogen, adipokines, and ultimately LBP. If successful, this work will provide new information and insights regarding the development of disc-disease and explore novel avenues for LBP treatment strategies in young females.
- Funding Agency
- Irish Research Council (IRC)
- Date From
- 2022
- Date To
- 2026
- Title
- CEMACUBE EIT Master's programme in Biomedical Engineering (2021)
- Summary
- Common European Masters in Biomedical Engineering (CEMACUBE) received the European Institute of Innovation and Technology (EIT) label, which was successfully awarded in 2019. The EIT is a European innovation body inspiring a new generation of entrepreneurs and innovators in Europe. This initiative links with the TCD MSc in Bioengineering with specialisation in Tissue Engineering.
- Funding Agency
- EIT Health
- Date From
- 2021
- Date To
- 2021
- Title
- Rejuvenation of the Intervertebral Disc Using Self-Healing Biomimetic Extracellular Matrix Biomaterial Tissue Adhesives (iDISC)
- Summary
- Lower back pain is a global epidemiological and socioeconomic problem. This project envisions a future whereby patients with degenerated intervertebral discs are injected with a self-healing biomimetic adhesive biomaterial which can restore both the biochemical and biomechanical properties to native tissue levels. Current surgical procedures do not replace herniated tissue from the central nucleus pulposus or repair the annulus fibrosus (outer ring of tissue), which can lead to accelerated degeneration, reherniation and recurrent pain. Spinal fusion, whereby the compromised or degenerated tissue is removed, and the vertebral segments are fused together, does not restore biomechanical function leading to degeneration of adjacent discs with long-term failure rates as high as 40%. My lab has developed a biomimetic injectable hydrogel (iDISC) consisting of the main components (collagen and chondroitin sulfate) of native disc tissue that can be tailored to match the biochemical and biomechanical properties of native disc tissue. In addition, the iDISC hydrogel demonstrates self-healing and adhesive properties to facilitate tissue integration and exhibits excellent cell biocompatibility. The objective of this proposal is to perform in depth in vitro characterisation (WP1), multiaxial biomechanical testing (WP2), pre-clinical evaluation (WP3) and marketing and commercialisation evaluation (WP4). The development of these injectable biomimetic hydrogel systems may facilitate earlier interventions aimed at halting the degenerative process, restore natural biomechanical function, enhancing patient accessibility, improving quality of life, reduce healthcare expenses and lost productivity in the European Union. The platform technology and knowledge generated through this research are beyond the current state-of-the-art and will provide a significant transformative scientific and clinical step change opening new horizons in minimally invasive spine treatment strategies.
- Funding Agency
- European Research Council (ERC)
- Title
- Consolidating the Expertise of Necmettin Erbakan University Towards the Development of Biofibers for Wound Healing and Tissue Regeneration (REGENEU)
- Summary
- The proposal REGENEU aims to meet the requirements of the HORIZON-WIDERA-2021-ACCESS-03-01: Twinning Call. The main focus of this project is to increase the scientific capabilities and excellence of NEU in the field of biofiber research and development for translational application. This target is supported by European partners TLC-RT (Germany), TERM (Germany) and AMBER of the University of Dublin, Trinity College Dublin (Ireland).
- Funding Agency
- HORIZON-CSA
- Date From
- 2022
- Date To
- 2024
- Title
- Personalised Medicine for Intervertebral Disc Regeneration- Integrating Profiling, Predictive Modelling and Gene Activated Biomaterials (INTEGRATE)
- Summary
- Lower back pain is a global epidemiological and socioeconomic problem. Biomaterial and cell-based therapies have been pursued for the treatment of degenerated intervertebral disc (IVD), with a number of clinical trials underway. However, the degenerated intervertebral disc has a distinct environment (e.g. altered oxygen, glucose, acidity, inflammatory cytokine levels) that is unique to an individual (i.e. patient-specific) and will ultimately determine the likelihood and rate at which regeneration can occur. A "one size fits all" approach will lead to the failure to demonstrate efficacy of advanced therapies, as they are not being designed or personalised for individual patients. This proposal envisions a future whereby advanced gene activated cell therapies are personalised (targeting regeneration or modulating inflammation) to treat back pain based on knowing the individuals unique disc microenvironment. This will be achieved through profiling of individual patient disc microenvironmental factors, with in vitro screening and in silico modelling to design cell therapies and predict regeneration outcomes (Aim 1) combined with the development of tailored functionalised gene activated biomaterials (Aim 2), to enhance matrix formation and modulate the inflammatory processes (Aim 3). Gene-based therapy offers several advantages over direct delivery of proteins or small molecules, among them the possibility of sustained efficacy and endogenous synthesis of growth factors or suppression of inflammatory factors and pathways. The platform technology (personalised gene activated biomaterials to regulate regeneration and inflammation) and knowledge (tailoring cell therapies to suit patient-specific microenvironments) generated through this research are beyond the current state-of-the-art and will provide a significant transformative scientific and clinical step change opening new horizons in minimally-invasive therapeutic strategies.
- Funding Agency
- European Research Council
- Date From
- 2020
- Date To
- 2025
- Title
- COVI3D - The development and characterization of lung ECM bioinks to 3D bioprint human lung tissue for use in the pre-clinical testing of novel COVID-19 therapies
- Summary
- The development of rapid, high-throughput, physiologically relevant models that can be used to better understand the pathophysiology of COVID-19 infection will be crucial in the race to develop COVID-19 therapeutics. While 2D in vitro cell culture systems allow for high-throughput analysis of therapeutics, they fail to model the complex lung environment; conversely animal models may better model the human system but are expensive, time-consuming to develop and low-throughput. Therefore, we propose to leverage our extensive knowledge of 3D bioprinting to develop a physiologically relevant and highly scaleable 3D model of lung tissue which can be combined with human cells to allow for accurate and rapid in vitro testing of potential COVID therapeutics. The overall aim of this project is to leverage our 3D bioprinting hardware and expertise in decellularized extracellular matrix (dECM) derived biomaterials to engineer lung tissue to support viral infectivity research and the search for an effective therapy against SARS-CoV-2, the novel coronavirus which causes COVID-19. Our specific objectives are to (1) develop and characterise a porcine lung extracellular matrix (ECM) bioink that can be used for the 3D bioprinting of lung tissue models, and (2) characterise the phenotype of lung cells within 3D bioprinted lung tissue. This 3D tissue model will then be shared with relevant COVID-19 researchers in Trinity.
- Funding Agency
- Trinity College Dublin
- Date From
- 2020
- Date To
- 2020
- Title
- 3D printing of fibrous electroconductive biomaterials with controlled architectures for peripheral nerve repair
- Summary
- Peripheral nerve injury remains a major clinical problem. Autografts are the current 'gold standard' but are hampered by limited availability of donor tissue with poor prognosis for functional recovery at both the donor and recipient sites. As a result, new approaches are currently being investigated to develop artificial nerve grafts which mimic the properties of autologous grafts. Additive manufacturing (AM) techniques such as 3D bioprinting (3DBP) offers exciting new opportunities and horizons to engineer nerve guidance conduits (NGCs) that more closely match the composition and structure of native nerve tissue. These approaches facilitate precise control over the external and internal microarchitecture geometry. In the context of developing engineered nerve tissue AM offers the added ability to incorporate drug delivery systems with tailorable spatial and temporal release profiles of individual drugs. Similarly, following peripheral nerve injury, the transfer of electrical signals across a damaged nerve is inhibited, resulting in degeneration of the distal nerve segment. Printing offers the potential to create specific geometries with defined micropatterns as well as incorporating electroconductive biomaterials to provide direct electrical activation to enhance tissue repair, enhance cell function and alignment. Applying electrical stimuli may enhance the regeneration as it is known that inhibition of electrical signalling can impede normal tissue function. Using bioinks derived from native nerve as a biomaterial platform developed in the Buckley lab for peripheral nerve tissue regeneration, this project proposes to enhance regenerative capacity of NGCs through the incorporation of electroconductive materials from partner labs in AMBER (e.g. graphene, silver nanowires, polypyrrole). Such a conductive biomaterial could thus provide direct electrical activation of isolated regions while also providing a scaffolding template to enhance the tissue repair process. Following optimisation of the fabrication/printing process, in vitro biocompatibility of the biofabricated NGCs and the response of cells to electrical stimuli will be assessed using in vitro methods prior to pre-clinical assessment.
- Funding Agency
- AMBER/SFI
- Date From
- 2021
- Date To
- 2025
- Title
- AMBER (Advanced Materials and Bioengineering Research) Centre Phase 2
- Summary
- This is funding to renew the AMBER centre. AMBER unites Ireland's leading scientists in Physics, Chemistry, Engineering and Medicine to deliver world class materials research with economic and societal impact in collaboration with industry.
- Funding Agency
- Science Foundation Ireland (SFI)
- Date From
- 2019
- Date To
- 2025
- Title
- CEMACUBE EIT Track
- Summary
- Common European Masters in Biomedical Engineering (CEMACUBE) received the European Institute of Innovation and Technology (EIT) label, which was successfully awarded in 2019. The EIT is a European innovation body inspiring a new generation of entrepreneurs and innovators in Europe. This initiative links with the TCD MSc in Bioengineering with specialisation in Tissue Engineering. Co-applicants: RWTH Aachen; Trinity College Dublin; Ghent University; University Medical Center Groningen
- Funding Agency
- EIT Health
- Date From
- 2019
- Date To
- 2020
- Title
- Transition- Delivering Innovative Materials for Medical Devices
- Summary
- Orthopaedic medicine involves treating conditions that affect the bones, soft tissue and joints. 3D printing has the potential to transform treatments in orthopaedic medicine and the orthopaedic device industry, enabling the development of personalised implants and accelerating the supply chain of device companies. TRANSITION aims to develop a hybrid device consisting of a titanium core (providing mechanical integrity) overlaid by a layer of functional tissue (engineered bone and articular cartilage) which will be particularly suited to hip and knee implants. In working towards this aim the project team will strive to advance the underpinning science and technology of metal, polymer and biological 3D printing as well as surface treatments and functional coatings. These advances will have direct benefits for improving existing implant technologies in parallel to the end goal. A key goal is to have a subset of products ready for regulatory submission and clinical studies by the end of the research programme.
- Funding Agency
- Science Foundation Ireland (SFI) Spokes Award, DePuy Synthes / Johnson and Johnson
- Date From
- Jan 2018
- Date To
- Jan 2023
- Title
- CEMACUBE EIT Track
- Summary
- Common European Masters in Biomedical Engineering (CEMACUBE) received the European Institute of Innovation and Technology (EIT) label, which was successfully awarded in 2019. The EIT is a European innovation body inspiring a new generation of entrepreneurs and innovators in Europe. This initiative links with the TCD MSc in Bioengineering with specialisation in Tissue Engineering. Co-applicants: RWTH Aachen; Trinity College Dublin; Ghent University; University Medical Center Groningen
- Funding Agency
- EIT Health
- Date From
- 2018
- Date To
- 2019
- Title
- A Bioactive Collagen-Based Conduit For Peripheral Nerve Repair
- Summary
- Peripheral nerve injury remains a major clinical problem. It is estimated that 5% of multiple trauma patients have root, plexus or peripheral nerve injuries. These injuries are typically caused by transection or burns or as a result of degenerative conditions such as amyotrophic lateral sclerosis, Parkinson's disease and multiple sclerosis. Ideally, prompt surgical intervention is required but if the injury size is >5mm or direct suturing is not possible, autografts are utilised and are currently the accepted clinical 'gold-standard' for treatment. However, autografts are hampered by limited availability of donor tissue and the relatively poor prognosis for functional recovery at both the donor and recipient sites. As a result, new approaches are currently being investigated to develop artificial nerve grafts which mimic the properties of autologous grafts. Commercially available nerve guidance conduits have been developed and present promising alternatives to autografts. It is estimated that the worldwide market for peripheral nerve repair conduits is over $100 million. However, challenges arising with their use have created a major gap in the market for a drug and cell free conduit that can facilitate repair of large defects. This is the focus of the current project which will be centred on two approaches which will be investigated in parallel: (i) the development of a bioactive collagen-based conduit containing inductive macromolecules and (ii) the development of a decellularized nerve graft.
- Funding Agency
- Science Foundation Ireland
- Date From
- 1 Mar2017
- Date To
- 28 Feb 2020
- Title
- Intraoperative Single Stage Procedure for Intervertebral Disc Regeneration (INSPIRE)
- Summary
- 97% of individuals over the age of 50 exhibit degeneration of the intervertebral disc (IVD). Recent investigations have shown that cell-based strategies can retard degenerative changes. However, the challenge for clinical translation remains in identifying suitable cell sources and biomaterial delivery systems to support such cells within the harsh and complex biochemical (low pH) and biomechanical environment of the disc. Intraoperative single stage cell therapies are highly attractive, avoiding the need for two hospital visits and costly in vitro cell expansion. In addition, developing injectable biomaterial systems is pivotal for minimally invasive therapeutic approaches. The overall aim of this proposal is to develop an autologous single stage therapy for intervertebral disc (IVD) regeneration. This consists of tissue harvesting from nasal septum, rapid cell isolation, development of biomimetic injectable cellular microcapsules (Aim 1) combined with pH buffering biomaterials to regulate the acidic microenvironment that exists in native disc tissue (Aim 2) and then evaluating the proposed strategy in both ex-vivo organ culture and in-vivo models (Aim 3). If successful, the strategies outlined will create a paradigm shift in which the scientific community and clinical investigators approach early stage disc degeneration.
- Funding Agency
- Science Foundation Ireland
- Date From
- Dec 2016
- Date To
- Dec 2020
- Title
- Towards a Single Stage Procedure for Intervertebral Disc Repair using Nasal Derived Chondrocytes (NasDisc)
- Summary
- Autologous Disc Cell Transplantation (ADCT) has been in clinical use for the treatment of degenerated IVD in a similar manner to those currently used for cartilage repair of the knee joint. Although these therapies have made it to the clinical setting there are significant concerns regarding its effectiveness due to issues of cell leakage following cell injection into the disc, the limited tissue forming capacity of culture expanded NP cells derived from degenerated tissue and questions as to whether the number of nucleus pulposus (NP) cells that can be isolated from a degenerated disc is sufficient to meet the requirements for successful treatment. Therefore, there is a need to identify an alternative cell source for the development and clinical translation of next generation therapies for IVD regeneration. The objective of this proposal is to determine the feasibility and the subsequent tissue forming capacity of freshly isolated nasal septal derived chondrocytes on porous microcarriers to augment tissue regeneration as part of a single stage procedure.
- Funding Agency
- Research Capacity Building Grant by TCD Research Committee
- Date From
- May 2015
- Date To
- May 2016
- Title
- Over-the-wire Drug Delivery Device
- Summary
- A recently developed and patented technology (Delivering drugs to desired locations within a mammal, PCT/US11/26040) at the Mayo Clinic may offer significant clinical and commercial opportunity to sustainably deliver pharmacologic agents over long periods of time. This is an Over-the-Wire Drug Delivery system that allows the placement of an anchored device on the posterior wall of the vertebral body, near the dorsal root ganglion and disc. This device could potentially provide a durable therapy and prolong the residence for short acting agents (e.g. Clonidine, dexmedetomidine, infliximab, etanercept). The objective of this project is to assess the commercial feasibility for further development and identify key tasks for continued investment.
- Funding Agency
- Enterprise Ireland
- Date From
- Apr 2015
- Date To
- Jan 2016
- Title
- Microencapsulation of Mesenchymal Stem Cells for Minimal Invasive Regeneration of the Intervertebral Disc
- Summary
- The primary objective of this proposal is to develop a biomimetic hydrogel that can be combined with cells to generate microbeads formed through electrohydrodynamic (EHD) spraying for minimal invasive delivery to the IVD. The second objective is to investigate if the combination of microencapsulated mesenchymal stem cells (MSCs) in a biomimetic hydrogel will augment or improve regeneration of the nucleus pulposus in an ex vivo full organ culture model of the IVD. The third and final objective of this proposal is to investigate the combined effects of biophysical stimuli and challenging biochemical nutrient environments that normally exist in vivo on the regenerative potential of microencapsulated MSCs. If successful, the approaches outlined will create a paradigm shift in the manner in which the scientific community and clinical investigators approach biological treatment of disc degeneration.
- Funding Agency
- Irish Research Council
- Date From
- Oct 2014
- Date To
- Oct 2018
- Title
- Injectable Biomimetic Hydrogels for Intervertebral Disc Regeneration (BIODISC)
- Summary
- : Support funding to facilitate the preparatory work leading to a proposal for the coordination of a research project (ERC-STG 2014) under the Horizon 2020 Programme.
- Funding Agency
- Enterprise Ireland
- Date From
- April 2014
- Date To
- Jun 2014
- Title
- Nucleus Pulposus Regeneration of the Intervertebral Disc (NuGenDisc)
- Summary
- Personal award to develop international collaborations with world-class renowned researchers, to, learn new techniques, and build on and establish new collaborations and networks. International laboratories: Prof. James Iatridis, School of Medicine at Mount Sinai in New York City and Prof. Benjamin Gattenbein, University of Bern, Switzerland.
- Funding Agency
- Irish Research Council
- Date From
- Mar 2014
- Date To
- Nov 2014
- Title
- Natural ECM Derived Injectable Microparticles for Regeneration of the Intervertebral Disc (DiscReGen)
- Summary
- Recent evidence suggests that degeneration initiates within the central region of the disc called the nucleus pulposus (NP). The objective of this grant is to develop an injectable microparticle encapsulated Extracellular Matrix (ECM) derived from NP tissue fabricated using electrospraying technology. It is hypothesised that these microparticles will provide key biochemical cues to stimulate repair and regeneration of the disc by the patient's own cells. This technology and approach for regeneration or repairing the intervertebral disc is highly attractive, and could be performed at an earlier stage of tissue degeneration to alleviate back pain thus reducing the number of patients requiring spinal fusion or artificial implant replacement surgery.
- Funding Agency
- Enterprise Ireland
- Date From
- Sep 2012
- Date To
- May 2014
- Title
- Injectable Hydrogel Composites for Stem Cell Repair of the Nucleus Pulposus
- Summary
- Autologous cell implantation therapies have been proposed for the treatment of degenerated IVD similar to those currently used for cartilage repair in the knee. Recent investigations have demonstrated that reimplantation of extracted nucleus pulposus (NP) cells can retard degenerative changes, and injection of autologous. NP cells have been clinically tested in humans with herniated discs. However, it is believed that the number of NP cells that can be isolated from a degenerated disc is insufficient to meet the requirements for successful treatment. As an alternative, mesenchymal stem cells (MSCs) possess significant potential and perhaps provide a more readily available and clinically feasible source of cells for IVD repair therapies. A key challenge with using MSCs is developing suitable biomaterials that can be injected into the NP compartment whilst providing the optimal biochemical and biophysical environment to facilitate differentiation. The proposed project will utilise state of the art tissue engineering and biomaterial strategies using a combination of MSCs, injectable growth factor releasing hydrogel based systems.
- Funding Agency
- Higher Education Authority (HEA).
- Date From
- Apr 2012
- Date To
- Apr 2016
- Title
- Isolation and characterization of different mesenchymal stem cell populations
- Summary
- To acquire expertise and training in the isolation, purification and characterisation of mesenchymal stem cells (MSCs) from different tissue sources (bone marrow, intramedullary adipose tissue, infrapatellar fat pad and synovium membrane) using magnetic bead separation technology and FACS. International Laboratory Collaborators: Prof. Dennis McGonagle and Dr. Elena Jones, Leeds Institute of Molecular Medicine, University of Leeds, United Kingdom.
- Funding Agency
- Science Foundation Ireland (SFI)
- Date From
- Apr 2009
- Date To
- Jan 2010
Recognition
Representations
International Advisory Review Board (ARB)- Journal of Orthopaedic Research-Spine.
External Examiner- MSc Tissue Engineering for Regenerative Medicine programme, University of Manchester
Advisory Board Member- Master's degree programme Biomedical Engineering University of Groningen, Netherlands.
CEMACUBE/KIC-EIT Board Member: University representation on the board of the Common European Masters in Biomedical Engineering (CEMACUBE). Buckley was a co-applicant for CEMACUBE to receive the European Institute of Innovation and Technology (EIT) label, which was successfully awarded in 2019. The EIT is a European innovation body inspiring a new generation of entrepreneurs and innovators in Europe.
Quality and Qualifications Ireland (QQI) panel expert. QQI is the national agency responsible for the National Framework of Qualifications (NFQ).
Awards and Honours
Top Cited Article, Journal of Orthopaedic Research-Spine (2022-2023). Consolidating and re-evaluating the human disc nutrient microenvironment. JOR Spine, 5(1):e1192, 2022. Contribution- Senior Author
Top Cited Article, Journal of Orthopaedic Research-Spine (2022-2023). Rat tail models for assessment of injectable nucleus pulposus regeneration strategies. JOR Spine, 5(3): e1216, 2022 Contribution- Senior Author
Top Downloaded Article, Journal of Orthopaedic Research-Spine (2022-2023). Rat tail models for assessment of injectable nucleus pulposus regeneration strategies. JOR Spine, 5(3): e1216, 2022 Contribution- Senior Author
Top Cited Article, Journal of Orthopaedic Research-Spine (2021-2022). Investigating the physiological relevance of ex vivo disc organ culture nutrient microenvironments using in-silico modelling and experimental validation. JOR Spine 4(2):e1141, 2021. Contribution- Senior Author
Trinity College Dublin Innovation Awards 2022- In recognition of achieving Campus Company Status (Altach Biomedical). Contribution- Co-Founder
Fellow of Trinity College Dublin (FTCD)
European Research Council Consolidator Grant- Principal Investigator
Top 10% Downloaded Articles, Journal of Orthopaedic Research-Spine (2018-2019). Advancing cell therapies for intervertebral disc regeneration from the lab to the clinic: Recommendations of the ORS spine section. Contribution- Senior Author
Top 10% Downloaded Articles, Journal of Biomedical Materials Research Part A (2018-2019). Glyoxal cross-linking of solubilized extracellular matrix to produce highly porous, elastic, and chondro-permissive scaffolds for orthopedic tissue engineering. Contribution- Co-Author
Top 20 Downloaded Articles, Journal of Orthopaedic Research-Spine (2017-2018). Critical aspects and challenges for intervertebral disc repair and regeneration-Harnessing advances in tissue engineering. Contribution- First Author
Top 20 Downloaded Articles, Journal of Orthopaedic Research-Spine (2017-2018). Advancing cell therapies for intervertebral disc regeneration from the lab to the clinic: Recommendations of the ORS spine section. Contribution- Senior Author
Honorary Associate Professor, Royal College of Surgeons in Ireland & Trinity College Dublin
Science Foundation Ireland, Career Development Award- Principal Investigator
Irish Research Council (IRC) New foundations Award- Principal Investigator
Global Medical Discovery Series. Contribution- Principal Investigator
Engineers Ireland Biomedical Research Medal- Co-Investigator
Best overall MSc Thesis in Bioengineering. Principal Investigator
Engineers Ireland Biomedical Research Medal. Co-Investigator
European Society of Biomechanics S.M. Perren Award. Co-Investigator
Science Foundation Ireland Travel Fellowship- Investigator
Memberships
Educating Students in Engineering and Medicine (ESEM), Co- Founding member and Treasurer
Tissue Engineering and Regenerative Medicine International Society (TERMIS)
European Society of Biomechanics (ESB)
Orthopaedic Research Society (ORS)