What do we have?
All microscopes are located in Room -2.46 in the Trinity Biomedical Sciences Institute.
Leica SP8 Gated STED
- Leica motorised inverted microscope.
- 10x and 20X dry objectives and 40X, 63X and 100X oil objectives.
- Resonant scanner for imaging at 24 frames per second.
- Galvoflow, SuperZ galvanometer.
- Okolabs stage top 37 degree incubator for live imaging.
- 592nm STED laser for super resolution imaging.
- 5 detectors with 3 PMTs and 2 HyD detectors.
- The light sources include 405nm diode, a multi argon laser and a white laser that can give up to 8 wavelengths simultaneously between 470nm and 670nm.
- Has both high resolution and flexibility to cover a wide range of experimental setups in fluorescent microscopy.
Leica SP8 confocal
- Leica inverted microscope.
- 10x dry objective and 40X, 63X oil objectives.
- 3 PMT detectors with prism based wavelength separation.
- Diode lasers at 405nm, 488nm, 552nm and 638nm.
Olympus FV1000 Point-Scanning Confocal Microscope
- Olympus IX81 motorised inverted microscope.
- Prior motorised stage.
- 10X, 20X and 40X dry and 40X and 63X oil objectives.
- Argon laser, multi-line 457 / 488/ 515 nm, GreenHelium-Neon laser 543 nm, Red Helium-Neon laser 633 nm, Near-Violet Laser diode 405 nm.
- 3 PMTs and 1 transmission light detector.
- Fitted with an incubator for live cell experiments.
Andor Spinning disk
- Olympus IX81 motorised inverted microscope.
- Piezo Z and motor XY Stage control.
- 10X, 20X dry and 40X and 63X oil objectives.
- PLAPON 60X Oil NA = 1.42, UV transparent.
- Yokogawa CSU -X1- A2 Spinning Disk Unit- with straight by-pass mode- Quick switch for cameras use with non-confocal modalities (e.g. DIC, epifluorescence and TIRF) without light loss.
- Andor Multi-Port Unit MPU - Utilize sharing wavelengths between Spinning Disk, FRAPPA and TIRF devices.
- FRAPPA unit-Fluorescence Recovery After Photo bleach (FRAP) and Photo Activation (PA).
- Diode lasers 405nm, 445 nm, 488 nm, 514 nm, 561 nm.
- CoolLED light source for epi-fluorescence at 445nm, 480nm and 560nm.
- Two Andor iXon+ ultra sensitive EMCCD cameras.
- Fitted with an incubator for live cell experiments.
Olympus BX51 upright microscope
- Olympus BX51 upright microscope.
- 4X, 10X, 20X, 40X and 60X dry objectives.
- LED light source with 365nm, 470nm, 535nm and 635nm and matching filter cubes.
- DP73 digital camera and CellSens software.
- Can be used for slides for brightfield, DIC and immunofluorescence.
Olympus IX81-long focal length Fluorescent microscope
- Olympus IX81 inverted microscope with motorised z stage.
- 4X, 10X, 20X, 40X and 60X long working distance dry objectives.
- Filter cube contains dichroics and filters for DAPI, GFP, YFP & Cy3 chromophores.
- DP71 digital camera and cell dimension imaging software.
- Can be used for both slides and plastic dishes for brightfield, phase contrast and immunofluorescence.
Nikon Eclipse E400 polarising microscope
- Nikon E400 upright polarizing microscope
- 10X, 20X and 40X dry objectives