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Image 1 Rhodopsin (rho) -/- knockout retina transduced with a mix of 2 recombinant adeno associated virus serotype 2/5 vectors (rAAV2/5) (rho and EGFP). Red: rhodopsin. Green: EGFP. Blue: DAPI. Only the outer nuclear layer is labeled specifically. All labelled cells are photoreceptors. EGFP is uniformly distributed in the cells and rho is mainly transported into the photoreceptor outer segments. Analysed 2 weeks postinjection. Credit: Arpad Palfi Image 2 Subretinal transplantation of retinal cells into a rho-/- mouse
Green: transplanted retinal cells isolated from EGFP mice
red: recoverin staining (photoreceptors)
blue: DAPI Credit: Marius Ader Image 3 Wild-type 168 Bacillus subtillus stained with DAPI (blue) and Nile red (membrane marker) Credit: Paola Bisicchia Image 4 Localization of tubulin (cyan) in bloodstream forms of T. brucei Credit: Orla Hanrahan Image 5 Localization of the VSG (red) and the nucleus and kinetoplast (blue) in bloodstream forms of T. brucei Credit: Orla Hanrahan Image 6 THP-1 cells, stained with MitoTracker Red (mitochondria), and cytochrome c labelled using Alexa 488 and nucleus stained using DRAQ-5. This image is a merge of all three Credit: Seonadh O’Leary Image 7 THP-1 cells, stained with MitoTracker Red (mitochondria), Apoptosis-inducing factor (AIF) labelled with Alexa 488 and the nucleus stained with DRAQ-5. This image is a merge of all three. Credit: Seonadh O’Leary Image 8 Mixed astrocyte/neuron culture from neonatal Wistar rats
Astrocytes are stained with anti Glial fibrillary acidic protein (GFAP), an intermediate filament specific to astrocytes (green) and neurons are stained with anti Tuj1, Neuron-specific class III beta-tubulin (red). DAPI is staining the nucleus in all cells (blue) Credit: Stephen Quinn