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Research > Cell Analysis Facilities > Microscopy and Imaging Centre > Common Fluorescent Probes
Common Fluorescent Probes
Spectral properties of similar dyes
Dye Name |
Colour |
Excitation Maxima |
Emission Maxima |
Hoechst/DAPI |
Blue |
350 nm |
461 nm |
ECFP |
Cyan |
439 nm |
476 nm |
EGFP |
Green |
484 nm |
507 nm |
Alexa 488 |
Green |
495 nm |
519 nm |
FITC |
Green |
495 nm |
521 nm |
DyLight 488 |
Green |
493 nm |
518 nm |
Cy2 dye |
Green |
492 nm |
510 nm |
EYFP |
Yellow |
514 nm |
527 nm |
DyLight 549 |
Yellow |
562 nm |
576 nm |
Alexa 555 |
Orange |
555 nm |
565 nm |
Cy3 dye |
Orange |
550 nm |
570 nm |
Tetra Methyl Rhodamine (TRITC) |
Orange |
550 nm |
570 nm |
Alexa 546 |
Orange |
556 nm |
573 nm |
TagRFP |
Red-Orange |
555 nm |
584 nm |
Rhodamine RedX |
Red-Orange |
570 nm |
590 nm |
Alexa 555 |
Red-Orange |
555 nm |
565 nm |
Alexa 568 |
Red-Orange |
578 nm |
603 nm |
mRFP1 |
Red-Orange |
584 nm |
607 nm |
DyLight 594 |
Red-Orange |
593 nm |
613 nm |
Alexa 594 |
Red |
590 nm |
617 nm |
DyLight 594 |
Red |
591 nm |
616 nm |
Texas Red |
Red |
596 nm |
620 nm |
Alexa 610 |
Red |
612 nm |
628 nm |
Alexa 633 |
Far Red |
632 nm |
647 nm |
Alexa 647 |
Far Red |
650 nm |
668 nm |
Cy5 |
Far Red |
649 nm |
670 nm |
DyLight 649 |
Far Red |
652 nm |
670 nm |