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You are here Research > Cell Analysis Facilities > Microscopy and Imaging Centre > Common Fluorescent Probes

Common Fluorescent Probes

Spectral properties of similar dyes

Dye Name Colour Excitation Maxima Emission Maxima
Hoechst/DAPI Blue 350 nm 461 nm
ECFP Cyan 439 nm 476 nm
EGFP Green 484 nm 507 nm
Alexa 488 Green 495 nm 519 nm
FITC Green 495 nm 521 nm
DyLight 488 Green 493 nm 518 nm
Cy2 dye Green 492 nm 510 nm
EYFP Yellow 514 nm 527 nm
DyLight 549 Yellow 562 nm 576 nm
Alexa 555 Orange 555 nm 565 nm
Cy3 dye Orange 550 nm 570 nm
Tetra Methyl Rhodamine (TRITC) Orange 550 nm 570 nm
Alexa 546 Orange 556 nm 573 nm
TagRFP Red-Orange 555 nm 584 nm
Rhodamine RedX Red-Orange 570 nm 590 nm
Alexa 555 Red-Orange 555 nm 565 nm
Alexa 568 Red-Orange 578 nm 603 nm
mRFP1 Red-Orange 584 nm 607 nm
DyLight 594 Red-Orange 593 nm 613 nm
Alexa 594 Red 590 nm 617 nm
DyLight 594 Red 591 nm 616 nm
Texas Red Red 596 nm 620 nm
Alexa 610 Red 612 nm 628 nm
Alexa 633 Far Red 632 nm 647 nm
Alexa 647 Far Red 650 nm 668 nm
Cy5 Far Red 649 nm 670 nm
DyLight 649 Far Red 652 nm 670 nm